Biofilms

Abstract

Most microorganisms on earth live in aggregates such as films, flocs, mats, granules or sludge – this form of life is referred to as ‘biofilms’, and it is involved in the biogeochemical cycles of all major elements including metals. Encased in a highly hydrophilic matrix of extracellular polymeric substances, biofilm organisms can develop stable microconsortia and complex interactions, resulting in features of multicellular organisms.

Keywords: biofilm; EPS; cell–cell communication; biofiltration; biofouling; microbially influenced corrosion

Figure 1.

Transitional episodes in biofilm development by Pseudomonas aeruginosa strain PAO1 examined by transmitted light microscopy. Each panel represents a distinct episode in biofilm development. (a) Reversible attachment. Initial event in biofilm development, bacteria are attached to substratum at cells pole (arrow). (b) Irreversible attachment. Cells were cemented to the substratum and formed nascent cell clusters (arrow) with all cells in contact with the substratum. (c) Maturation‐1. Cell clusters matured (arrow) and were several cells thick, embedded in the EPS matrix. (d) Maturation‐2. Cell clusters reached maximum thickness, approximately 100 μm. (e and f) Dispersion. Cells evacuated interior portions of cell clusters (arrow), forming void spaces (figure supplied by D. Davies, Binghamton University). Reproduced with permission from Sauer et al..

Figure 2.

Scanning electron micrograph of a biofilm of Pseudomonas putida on a mineral surface. EPS (dehydrated for SEM sample preparation) are surrounding the cells, keeping them together and on the surface.

Figure 3.

Structures of quorum‐sensing signals and their derivatives. Letter designations for the Gram‐positive peptide signals indicate amino acids. For the Lactobacillus lactis signal nisin, the structural abbreviations were Bu, dehydroxybutyrine with a lanthionine bridge; Ha, dehydroalanine; Hb, dehydrobutirine (from Horswill et al., , with kind permission of Springer Science and Business Media; figure supplied by Alexander R. Horswill, University of Iowa and Matthew R Parsek, University of Washington).

Figure 4.

Artist's view of biofilm architecture and processes. Reproduced by permission of P Dirckx, Center for Biofilm Engineering, MSU, Bozeman.

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Further Reading

Costerton JW (2007) The Biofilm Primer. New York: Springer.

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Ehrlich HL (2002) Geomicrobiology. New York: Marcel Dekker.

Flemming H‐C, Szewzyk U and Griebe T (eds) (2000) Biofilms. Investigative Methods and Applications. Lancaster: Technomic Publications.

Ghannoum M and O'Toole GA (eds) (2004) Microbial Biofilms. Washington: ASM Press.

Jass J, Surman S and Walker J (eds) (2003) Medical Biofilms. Detection, Prevention and Control. London: Wiley.

Kjelleberg S and Givskov M (eds) (2007) The biofilm mode of life. Horizon Bioscience. Wymondham, UK.

Krumbein WE, Paterson DM and Zavarzin GA (eds) (2003) Fossil and Recent Biofilms. Dordrecht: Kluwer Academic Publications.

Lewandowski Z and Givskov M (2007) Fundamentals of Biofilm Research. Boca Raton: CRC Press, Taylor & Francis.

Wilson M and Devine D (eds) (2003) Medical Implications of Biofilms. Cambridge: Cambridge University Press.

Wingender J, Neu TR and Flemming H‐C (eds) (1999) Microbial Extracellular Polymeric Substances. Heidelberg: Springer.

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How to Cite close
Flemming, Hans‐Curt(Apr 2008) Biofilms. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0000342.pub2]