Neurospora Genetics

Abstract

Neurospora crassa is a filamentous fungus of the class Ascomycetes. It is a genetically well‐understood organism used in the original work that defined the relationship between genes and enzymes.

Keywords: Neurospora; tetrad analysis; gene–enzyme relationships; ascomycete; genome organization

Figure 1.

Asexual (inner) and sexual (outer) cycles of N. crassa. The sexual spores, or conidia, function in the asexual cycle as vegetative propagules, and in the sexual cycle as fertilizing agents. All matings require the participation of both mating types, A and a, but either mating type may function as the male or female parent. In the sexual cycle, the diameter of the protoperithecium (bottom) is about one‐fifth or less of the diameter of the mature perithecium (top). The trichogyne shown protruding from the protoperithecium is shown being fertilized by a conidium of the opposite mating type. (Adapted from Davis and de Serres, . Copyright © 1970 Academic Press.)

Figure 2.

The meiotic process in N. crassa, starting with haploid gametic cells AB and ab. The fusion that takes place in the ascus initial (a and b, right) is followed by two meiotic (c and d, right) and a final mitotic (e, right) division. At metaphase of the first division, the two pairs of homologous chromosomes (bivalents) may line up such that A will segregate with B (case 1), or with b (case 2), leading to PD or NPD tetrads, respectively. In case 3, a crossover between gene B/b and the centromere of the long chromosome leads to delayed segregation of the B/b allele pair, such that a tetratype (T) tetrad is formed. Each meiotic product divides once before spore walls form around the eight nuclei in the linear ascus (f, right). (Adapted from Davis and de Serres, . Copyright © 1970 Academic Press.)

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References

Beadle GW and Tatum EL (1941) Genetic control of biochemical reactions in Neurospora. Proceedings of the National Academy of Sciences of the USA 27: 499–506.

Beadle GW and Tatum EL (1945) Neurospora II. Methods of producing and detecting mutations concerned with nutritional requirements. American Journal of Botany 32: 678–686.

Borkovich KA, Alex LA, Yarden O et al. (2004) Lessons from the genome sequence of Neurospora crassa: tracing the path from genomic blueprint to multicellular organism. Microbiology and Molecular Biology Reviews 68: 1–108.

Cogoni C (2001) Homology‐dependent gene silencing mechanisms in fungi. Annual Review of Microbiology 55: 381–406.

Davis RH (2000) Neurospora. Contributions of a Model Organism. New York: Oxford University Press.

Davis RH and de Serres FJ (1970) Genetic and microbiological methods for Neurospora crassa. Methods in Enzymology 17(A): 79–143.

Gaertner FH (1978) Catalytic and structural properties of the pentafunctional arom enzyme conjugate. In: Srere PA and Estabrook RW (eds) Microenvironments and Metabolic Compartmentation, pp. 345–353. New York: Academic Press

Glass NL and Kuldau GA (1992) Mating type and vegetative incompatibility in filamentous ascomycetes. Annual Review of Phytopathology 30: 201–224.

Horowitz NH and Leupold U (1951) Some recent studies bearing on the one gene–one enzyme hypothesis. Cold Spring Harbor Symposia on Quantitative Biology XVI: 65–74.

Mitchell MB (1955) Aberrant recombination of pyridoxine mutants of Neurospora. Proceedings of the National Academy of Sciences of the USA 41: 215–220.

Orbach MJ (1992) Untitled note in Fungal Genetics Newsletter 39: 92.

Orbach MJ, Vollrath D and Davis RW (1988) An electrophoretic karyotype of Neurospora crassa. Molecular and Cellular Biology 8: 1469–1473.

Orr‐Weaver TL and Szostak J (1985) Fungal recombination. Microbiological Reviews 49: 33–58.

Perkins DD, Radford A and Sachs MS (2001) The Neurospora compendium. Chromosomal Loci. New York: Academic Press.

Shear CL and Dodge BO (1927) Life histories and heterothallism of the red bread‐mold fungi of the Monilia sitophila group. Journal of Agricultural Research 34: 1019–1042.

Shiu PK and Metzenberg RL (2002) Meiotic silencing by unpaired DNA: properties, regulation and suppression. Genetics 161: 1483–1495.

Srb AM and Horowitz NH (1944) The ornithine cycle in Neurospora and its genetic control. Journal of Biological Chemistry 154: 129–139.

Further Reading

Davis RH (2003) The Microbial Models of Molecular Biology. New York: Oxford University Press.

Davis RH (2004) Genetics of Neurospora. Kuck U (ed.) The Mycota II. Genetics and Biotechnology, 2nd ed., pp. 3–18. Berlin: Springer

Davis RH and Perkins DD (2002) Neurospora: a model of model microbes. Nature Reviews Genetics 3: 7–13.

Horowitz NH (1991) Fifty years ago: the Neurospora revolution. Genetics 127: 631–635.

Perkins DD (1992) Neurospora: the organism behind the molecular revolution. Genetics 130: 687–701.

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Davis, Rowland H(Apr 2006) Neurospora Genetics. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0004236]