Ribosomal Proteins in Eukaryotes


About 80 different ribosomal proteins are involved in the biogenesis and functioning of eukaryotic ribosomes. Genome sequencing efforts have contributed to our knowledge on their evolution, and recent progress in the structural analysis of the ribosome has considerably improved our understanding of the localization and function of individual ribosomal proteins, some of which are also involved in extraribosomal functions.

Keywords: eukaryotic ribosomal proteins; evolution; localization; function; genes

Figure 1.

The mammalian 40S ribosomal subunit model obtained by immuno‐electron microscopy in four different positions, showing (a) the location of ribosomal proteins S2, S3, S3A, S5, S7, S9, S13, S17, S19, S21 and S24, and (b) the location of initiation factors eIF2 and eIF3 with mRNA and Met‐tRNA (bound to eIF2) at the surface of the 40S ribosomal subunit. Views (top to bottom) are on the concave frontal side, the external side, the convex rear side and the interface side. Reproduced with permission from Lutsch et al. (1990), Copyright 1990, Gustav Fischer Verlag.



Arnold RJ, Polevoda B, Reilly JP and Sherman F (1999) The action of N‐terminal acetyltransferases on yeast ribosomal proteins. Journal of Biological Chemistry 274: 37035–37040.

Ballesta JP, Rodriguez‐Gabriel MA, Bou G et al. (1999) Phosphorylation of the yeast ribosomal stalk. Functional effects and enzymes involved in the process. (Review) FEMS Microbiological Review 23: 537–550.

Beckmann R, Spahn CM, Eswar N et al. (2001) Architecture of the protein‐conducting channel associated with the translating 80S ribosome. Cell 107: 361–372.

Bielka H (ed.) (1982) The Eukaryotic Ribosome. Berlin: Springer‐Verlag.

Bommer UA, Lutsch G, Stahl J and Bielka H (1991) Eukaryotic initiation factors eIF‐2 and eIF‐3: interactions, structure and localization in ribosomal initiation complexes. (Review) Biochimie 73: 1007–1019.

Clemens MJ and Bommer U‐A (1999) Translational control: the cancer connection. International Journal of Biochemistry and Cell Biology 31: 1–23.

Cui K, Coutts M, Stahl J and Sytkowski AJ (2000) Novel interaction between the transcription factor CHOP (GADD153) and the ribosomal protein FTE/S3a modulates erythropoiesis. Journal of Biological Chemistry 275: 7591–7596.

Dresios J, Panopoulos P, Suzuki K and Synetos D (2003) A dispensable yeast ribosomal protein optimises peptidyltransferase activity and affects translocation. Journal of Biological Chemistry 278: 3314–3322.

Fukushi S, Okada M, Stahl J et al. (2001) Ribosomal protein S5 interacts with the internal ribosomal entry site of hepatitis C virus. Journal of Biological Chemistry 276: 20824–20826.

Gomez‐Lorenzo MG, Spahn CM, Agrawal RK et al. (2000) Three‐dimensional cryo‐electron microscopy localization of EF2 in the Saccharomyces cerevisiae 80S ribosome at 17.5 Å resolution. EMBO Journal 19: 2710–2718.

Halic M, Becker T, Pool MR et al. (2004) Structure of the signal recognition particle interacting with the elongation‐arrested ribosome. Nature 427: 808–814.

Hu ZB, Minden MD, McCulloch EA and Stahl J (2000) Regulation of drug sensitivity by ribosomal protein S3a. Blood 95: 1047–1055.

Jang CY, Lee JY and Kim J (2004) RpS3, a DNA repair endonuclease and ribosomal protein, is involved in apoptosis. FEBS Letters 560: 81–85.

Lhoest J, Lobet Y, Costers E and Colson C (1984) Methylated proteins and amino acids in the ribosomes of Saccharomyces cerevisiae. European Journal of Biochemistry 141: 585–590.

Lutsch G, Stahl J, Kärgel HJ, Noll F and Bielka H (1990) Immunoelectron microscopic studies on the location of ribosomal proteins on the surface of the 40S ribosomal subunit from rat liver. European Journal of Cell Biology 51: 140–150.

Mazumder B, Sampath P, Seshadri V et al. (2003) Regulated release of L13a from the 60S ribosomal subunit as a mechanism of transcript‐specific translational control. Cell 115: 187–198.

Meyuhas O (2000) Synthesis of the translational apparatus is regulated at the translational level. (Review) European Journal of Biochemistry 267: 6321–6330.

Morgan DG, Menetret JF, Radermacher M et al. (2000) A comparison of the yeast and rabbit 80 S ribosome reveals the topology of the nascent chain exit tunnel, inter‐subunit bridges and mammalian rRNA expansion segments. Journal of Molecular Biology 301: 301–321.

Morgan DG, Menetret JF, Neuhof A, Rapoport TA and Akey CW (2002) Structure of the mammalian ribosome‐channel complex at 17Å resolution. Journal of Molecular Biology 324: 871–886.

Naoro H and Naoro H (1999) Involvement of ribosomal proteins in regulating cell growth and apoptosis: translational modulation or recruitment for extraribosomal activity? Immunology and Cell Biology 77: 197–205.

Neumann F and Krawinkel U (1997) Constitutive expression of human ribosomal protein L7 arrests the cell cycle in G1 and induces apoptosis in Jurkat T‐lymphoma cells. Experimental Cell Research 230: 252–261.

Odintsova TI, Muller EC, Ivanov AV et al. (2003) Characterization and analysis of post‐translational modifications of the human large cytoplasmic ribosomal subunit proteins by mass spectrometry and Edman sequencing. Journal of Protein Chemistry 22: 249–258.

Orfali KA, Ohene‐Abuakwa Y and Ball SE (2004) Diamond Blackfan anaemia in the UK: clinical and genetic heterogeneity. British Journal of Haematology 125: 243–252.

Pende M, Um SH, Mieulet V et al. (2004) S6K1(‐/‐)/S6K2(‐/‐) mice exhibit perinatal lethality and rapamycin‐sensitive 5′‐terminal oligopyrimidine mRNA translation and reveal a mitogen‐activated protein kinase‐dependent S6 kinase pathway. Molecular Cell Biology 24: 3112–3124.

Song D, Sakamoto S and Taniguchi T (2002) Inhibition of poly(ADP‐ribose) polymerase activity by Bcl‐2 in association with the ribosomal protein S3a. Biochemistry 41: 929–934.

Spahn CM, Beckmann R, Eswar N et al. (2001a) Structure of the 80S ribosome from Saccharomyces cerevisiae: tRNA‐ribosome and subunit–subunit interactions. Cell 107: 373–386.

Spahn CM, Kieft JS, Grassucci RA et al. (2001b) Hepatitis C virus IRES RNA‐induced changes in the conformation of the 40S ribosomal subunit. Science 291: 1959–1962.

Spence J, Gali RR, Dittmar G et al. (2000) Cell cycle‐regulated modification of the ribosome by a variant multiubiquitin chain. Cell 102: 67–76.

Stolovich M, Tang H, Hornstein E et al. (2002) Transduction of growth or mitogenic signals into translational activation of TOP mRNAs is fully reliant on the phosphatidylinositol 3‐kinase‐mediated pathway but requires neither S6K1 nor rpS6 phosphorylation. Molecular Cell Biology 22: 8101–8113.

Thomas G (2002) The S6 kinase signaling pathway in the control of development and growth. Biological Research 35: 305–313.

Uechi T, Tanaka T and Kenmochi N (2001) A complete map of the human ribosomal protein genes: assignment of 80 genes to the cytogenetic map and implications for human disorders. Genomics 72: 223–230.

Uechi T, Maeda N, Tanaka T and Kenmochi N (2002) Functional second genes generated by retrotransposition of the X‐linked ribosomal protein genes. Nucleic Acids Research 30: 5369–5375.

Warner JR, Vilardell J and Sohn JH (2001) Economics of ribosome biosynthesis. Cold Spring Harbor Symposia on Quantitative Biology 66: 567–574.

Wool IG, Chan YL and Glück A (1995) Structure and evolution of mammalian ribosomal proteins. Biochemistry and Cell Biology 73: 933–947.

Yoshihama M, Uechi T, Asakawa S et al. (2002) The human ribosomal protein genes: sequencing and comparative analysis of 73 genes. Genome Research 12: 379–390.

Zhang Z, Harrison P and Gerstein M (2002) Identification and analysis of over 2000 ribosomal protein pseudogenes in the human genome. Genome Research 12: 1466–1482.

Further Reading

The Ribosome (2001). Cold Spring Harbor Symposia on Quantitative Biology, Vol. LXVI.

Wool IG (1996) Extraribosomal functions of ribosomal proteins. Trends in Biochemical Sciences 21: 164–165.

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Bommer, Ulrich‐Axel, and Stahl, Joachim(Jan 2006) Ribosomal Proteins in Eukaryotes. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003867]