Bacterial Ribosomes: Assembly

Abstract

A ribosome consists of 50–70 different components and is, therefore, one of the most complicated structures known in biology. The large number of components requires a highly coordinated synthesis and assembly.

Keywords: translation; assembly gradient; assembly maps; reconstitution; ribosomal

Figure 1.

5′ and 3′ ends of 16S rRNA within the 30S subunit (a) and of 23S rRNA within the 50S subunit (b) viewed from the interface. (a) 30S subunit; Thermus thermophilus 30S (pdb 1fka): Ribbon representation of rRNA with the 5′ and 3′ ends of the 16S rRNA coloured green and red, respectively. The residues U6 (green) and C1512 (red) are in spacefill and are approximately 80 Å apart. (b) 50S subunit; Deinococcus radiodurans 50S (1kpj): Ribbon representation of rRNA with the 5′and 3′ ends of the 23S rRNA coloured green and red, respectively. The residues G1 (green) and A2877 (red) are shown in spacefill and are in contact with one another.

Figure 2.

Translational regulation of the r‐proteins. Usually the second or third cistron of a polycistronic mRNA codes for an rRNA‐binding protein which can also bind to the region of the ribosomal‐binding site of the first cistron of its own mRNA, thus competing with initiating 30S subunits. Therefore, this regulatory protein will inhibit the translation of its own polycistronic mRNA when a significant free pool of this protein is in the cell. Nine regulatory r‐proteins have been identified: S4, S7, S8, S15, S20, L1, L4, L10 and L20.

Figure 3.

The core structure (red lines) common to all 16S‐type rRNAs from ribosomes of various organisms. (a) E. coli; (b) Halobacterium volcanii; (c) yeast, cytoplasmic ribosomes; and (d) mitochondria of plants (maize). From Gutell RR, Weiser B, Woese CR and Noller HF (1985) Comparative anatomy of 16S‐like ribosomal RNA. Progress in Nucleic Acid Research and Molecular Biology32: 155–216.

Figure 4.

Assembly maps of the ribosome from E. coli. (a) The small 30S subunit. Proteins boxed in are those either required for the formation of RI30* particles or found in the isolated 21S particles. Modified from Held WA, Ballou B, Mizushima S and Nomura M (1974) Journal of Biological Chemistry249: 3103–3111. (b) The large 50S subunit. The three main fragments of 23S rRNA (13S, 8S and 12S) are indicated, and proteins are arranged according to their binding regions on 23S rRNA. The proteins boxed in are required for the transition RI50(1) → RI50(1)* of the early assembly. Proteins above the blue dotted line are those found in the RI50(1) particles. L5, L15 and L18, in the triangle, are the proteins important for mediating the binding of 5S rRNA to 23S rRNA. Modified from Herold M and Nierhaus KH (1987) Journal of Biological Chemistry262: 8826–8833.

Figure 5.

Ribosomal secondary and tertiary structures within the ribosome; the domains are marked with the same colour in the secondary (a, c) and the tertiary structures (b, d). (a) Secondary structure of T. thermophilus 16S rRNA, with its 5′, central, 3′‐major and 3′‐minor domains shaded in blue, magenta, red and yellow, respectively. (b) Three‐dimensional fold of 16S rRNA in 70S ribosomes, with its domains coloured as in (a). (c) Secondary structures of T. thermophilus 23S and 5S rRNAs, indicating domains I (blue), II (cyan), III (green), IV (yellow), V (red) and VI (magenta) of 23S rRNA. The rRNAs are numbered according to E. coli. (d) Three‐dimensional folds of 23S and 5S rRNAs, with their domains coloured as in (c). Modified from Yusupov MM et al. (2001) Science292: 883–896.

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References

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Further Reading

Culver GM (2003) Assembly of the 30S ribosomal subunit. Biopolymers 68: 234–249.

Lindahl L (1975) Intermediates and time kinetics to the in vivoassembly of Escherichia coliribosomes. Journal of Molecular Biology 92: 15–37.

Nierhaus KH (1990) The assembly of prokaryotic ribosomes. Biochimie 73: 739–755.

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Pace RP and Burgin AB (1990) Processing and evolution of the rRNAs. In: Hill WE (ed.) The Ribosome: Structure, Function and Evolution, pp. 417–425. Washington, DC: ASM Press.

Srivastava AK and Schlessinger D (1990) rRNA processing in Escherichia coli. In: Hill WE (ed.) The Ribosome: Structure, Function and Evolution, pp. 426–434. Washington, DC: ASM Press.

Traub P and Nomura M (1968) Structure and function of E. coli ribosomes. V. Reconstitution of functionally active 30S ribosomal particles from RNA and protein. Proceedings of the National Academy of Sciences of the USA 59: 777–784.

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Nierhaus, Knud H(Jan 2006) Bacterial Ribosomes: Assembly. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003947]