Nucleotide Excision Repair in Bacteria

Abstract

Nucleotide excision repair removes a wide variety of damages from bacterial genomes through a set of coordinated enzymatic reactions carried out by multiprotein complexes. It is coupled to other cellular processes, notably transcription.

Keywords: bacteria; enzymology of DNA repair; damage recognition; UvrABC endonuclease

Figure 1.

Nucleotide excision repair pathway in E. coli. UvrA2 dimer binds to undamaged DNA regions. UvrB then binds and the UvrA2B1 complex translocates to the damaged site (green circle). The RNAP–promoter complex can provide sites of preferential landing for two UvrA2B1. UvrA dissociates and UvrC binds to the UvrB–DNA complex at a damaged site. Dual incision is catalysed by UvrBC. UvrD displaces UvrC and an oligonucleotide containing the damage. DNA polymerase I fills the single‐stranded gap, and DNA ligase seals the nick.

Figure 2.

Strand selectivity by the UvrAB helicase. RNAP (α2, β, β′ and σ subunits) provides the UvrA2B1 complex with a preferred binding site at a promoter region (NER start site), because the association constant of the UvrA2B1 with unwound/noncomplementary DNA regions (bubbles) is ∼100‐fold higher than with duplex/complementary regions (Reproduced from Ahn and Grossman, Copyright © 1996 American Society for Biochemistry and Molecular Biology). Due to 5′–3′ directionality of its helicase activity UvrA2B1 can translocate productively only along the nontranscribed strand (NTS). During translocation the DNA damage‐recognition domain of the UvrA senses damaged sites on the transcribed strand (TS) which is selectively nicked when damage is found.

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References

Ahn B and Grossman L (1996) RNA polymerase signals UvrAB landing sites. Journal of Biological Chemistry 271: 21453–21461.

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Further Reading

Friedberg EC, Walker GC and Siede W (1995) DNA Repair and Mutagenesis. Washington, DC: ASM Press.

Grossman L and Thiagalingam S (1993) Nucleotide excision repair, a tracking mechanism in search of damage. Journal of Biological Chemistry 268: 16871–16874.

Grossman L, Lin CG, Ahn B (1998) Nucleotide excision repair in Escherichia coli. In: Nickoloff JA and Hoekstra MF (eds) DNA Damage and Repair, Vol. 1, pp. 11–27. Totowa, NJ: Humana Press.

Sancar A (1996) DNA excision repair. Annual Review of Biochemistry 65: 43–81.

Van Houten B (1990) Nucleotide excision repair in Escherichia coli. Microbiological Reviews 54: 18–51.

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How to Cite close
Grossman, Lawrence, and Kovalsky, Oleg(Dec 2001) Nucleotide Excision Repair in Bacteria. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0000560]