RecFOR Protein


In Escherichia coli the recF, recO and recR (recFOR) proteins interact with DNA, single‐stranded DNA‐binding (SSB) protein and RecA protein to facilitate recombinational repair (also called post‐replication repair and daughter‐strand gap repair) of DNA damage encountered by a DNA replication fork. The recF, recO and or recR genes have been found in 36 different types of bacteria ranging from the kingdoms of proteobacteria to aquificales.

Keywords: replication; repair; recombination; DNA; gene regulation; phylogeny

Figure 1.

This shows a diagram of the Escherichia coli chromosome and the approximate locations of the regions encoding the recFOR genes. Adjacent to the diagram of the chromosome are drawings of the genes around the recFOR gene. RecR region: dnaZX codes for the γ and τ clamp loading subunits of DNA Pol III holoenzyme and orf12 is an open reading frame for a protein of unknown function. RecO region: rnc encodes RNAase III; era is an essential gene of unknown function. RecF region: dnaA is essential for regulation and initiation of DNA replication at oriC. dnaN forms the β‐clamp or processivity factor for Pol III holoenzyme. gyrB is the β subunit of DNA gyrase.

Figure 2.

Model of RecFOR in postreplication repair. In the first panel, a replication fork approaching a noncoding lesion (small black box on the DNA) is diagrammed. In the second panel, the replication fork has translocated one Okazaki fragment past the noncoding lesion. No replication has occurred on the template strand containing the noncoding lesion. The remainder of the diagram is explained in the text.



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Sandler, Steven J(Apr 2001) RecFOR Protein. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1038/npg.els.0000588]