Transposases and Integrases

Abstract

Transposases and integrases are the proteins that mediate transposition reactions, i.e. the recombination reaction in which discrete DNA segments move between nonhomologous sites.

Keywords: transposition; recombination

Figure 1.

Cut and paste transposition.

Figure 2.

Donor cleavage.

Figure 3.

Retroviral integration.

Figure 4.

Bacteriophage Mu.

Figure 5.

Phosphoryl transfers in transposition.

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Further Reading

Beall EL and Rio DC (1996) Drosphilia IRBP/Ku p70 corresponds to the mutagen‐sensitive mus309 gene and is involved in P‐element excision in vivo. Genes and Development 10: 921–933.

Beall EL and Rio DC (1998) Transposase makes critical contacts with, and is stimulated by, single‐stranded DNA at the P element termini in vitro. EMBO Journal 17: 2122–2136.

Becker HA and Kunze R (1997) Maize Activator transposase has a bipartite DNA binding domain that recognizes subterminal sequences and the terminal inverted repeats. Molecular and General Genetics 254: 219–230.

Capy P, Langin T, Higuet D, Maurer P and Bazin C (1997) Do the integrases of LTR‐retrotransposons and class II element transposases have a common ancestor? Genetica 100: 63–72.

Craig NL (1995) Unity in transposition reactions. Science 270: 253–254.

Craig NL (1997) Target site selection in transposition. Annual Review of Biochemistry 66: 437–474.

Craig NL, Craigie R, Gellert M and Lambowitz A (eds) (in press) Mobile DNA II. Washington, DC: American Society for Microbiology.

Grindley NDF and Leschziner AE (1995) DNA transposition: from a black box to a color monitor. Cell 83: 1063–1066.

Haren L, Ton‐Hoang B and Chandler M (1999) Integrating DNA: transposases and retroviral integrases. Annual Review of Microbiology 53: 245–281.

Hickman AB, Li Y, Mathew SV et al. (2000) Unexpected structural diversity in DNA recombination: the restriction endonuclease connection. Molecular Cell 5: 1025–1034.

Kennedy AK, Hanniford DB and Mizuuchi K (2000) Single active site catalysis of the successive phosphoryl transfer steps by DNA transposases: insights form phosphorothioate stereoselectivity. Cell 101: 295–305.

Klobutcher LA and Herrick G (1997) Developmental genome reorganization in ciliated protozoa: the transposon link. Progress in Nucleic Acid Research and Molecular Biology 56: 1–62.

Lavoie BD, Shaw GS, Millner A and Chaconas G (1996) Anatomy of a flexer DNA complex inside a higher‐order transposition intermediate. Cell 85: 761–771.

Mahillon J and Chandler M (1998) Insertion sequences. Microbiology and Molecular Biology Review 62: 725–774.

Plasterk RH, Izsvak Z and Ivics Z (1999) Resident aliens: the Tc1/mariner superfamily of transposable elements. Trends in Genetics 15: 326–332.

Reznikoff WS, Bhasin A, Davies DR et al. (1999) Tn5: a molecular window on transposition. Biochemical and Biophysical Research Communications 266: 729–734.

Roth DB and Craig NL (1998) VDJ recombination: a transposase goes to work. Cell 94: 1–20.

Sakai JS, Kleckner N, Yang X and Guhathakurta A (2000) Tn10 transpososome assembly involves a folded intermediate that must be unfolded for target capture and strand transfer. EMBO Journal 19: 776–785.

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How to Cite close
Craig, Nancy L(Jun 2001) Transposases and Integrases. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0000593]