Transposases and integrases are the proteins that mediate transposition reactions, i.e. the recombination reaction in which discrete DNA segments move between nonhomologous sites.
Keywords: transposition; recombination
Transposases and Integrases
Nancy L Craig, Johns Hopkins School of Medicine, Baltimore, Maryland, USA
Published online: June 2001
DOI: 10.1038/npg.els.0000593
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Figure 1. Cut and paste transposition.
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Figure 2. Donor cleavage.
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Figure 3. Retroviral integration.
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Figure 4. Bacteriophage Mu.
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Figure 5. Phosphoryl transfers in transposition.
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| Further Reading | |
| Beall EL and Rio DC (1996) Drosphilia IRBP/Ku p70 corresponds to the mutagen-sensitive mus309 gene and is involved in P-element excision in vivo. Genes and Development 10: 921–933. | |
| Beall EL and Rio DC (1998) Transposase makes critical contacts with, and is stimulated by, single-stranded DNA at the P element termini in vitro. EMBO Journal 17: 2122–2136. | |
| Becker HA and Kunze R (1997) Maize Activator transposase has a bipartite DNA binding domain that recognizes subterminal sequences and the terminal inverted repeats. Molecular and General Genetics 254: 219–230. | |
| Capy P, Langin T, Higuet D, Maurer P and Bazin C (1997) Do the integrases of LTR-retrotransposons and class II element transposases have a common ancestor? Genetica 100: 63–72. | |
| Craig NL (1995) Unity in transposition reactions. Science 270: 253–254. | |
| Craig NL (1997) Target site selection in transposition. Annual Review of Biochemistry 66: 437–474. | |
| book Craig NL, Craigie R, Gellert M and Lambowitz A (eds) (in press) Mobile DNA II. Washington, DC: American Society for Microbiology. | |
| Grindley NDF and Leschziner AE (1995) DNA transposition: from a black box to a color monitor. Cell 83: 1063–1066. | |
| Haren L, Ton-Hoang B and Chandler M (1999) Integrating DNA: transposases and retroviral integrases. Annual Review of Microbiology 53: 245–281. | |
| Hickman AB, Li Y, Mathew SV et al. (2000) Unexpected structural diversity in DNA recombination: the restriction endonuclease connection. Molecular Cell 5: 1025–1034. | |
| Kennedy AK, Hanniford DB and Mizuuchi K (2000) Single active site catalysis of the successive phosphoryl transfer steps by DNA transposases: insights form phosphorothioate stereoselectivity. Cell 101: 295–305. | |
| Klobutcher LA and Herrick G (1997) Developmental genome reorganization in ciliated protozoa: the transposon link. Progress in Nucleic Acid Research and Molecular Biology 56: 1–62. | |
| Lavoie BD, Shaw GS, Millner A and Chaconas G (1996) Anatomy of a flexer DNA complex inside a higher-order transposition intermediate. Cell 85: 761–771. | |
| Mahillon J and Chandler M (1998) Insertion sequences. Microbiology and Molecular Biology Review 62: 725–774. | |
| Plasterk RH, Izsvak Z and Ivics Z (1999) Resident aliens: the Tc1/mariner superfamily of transposable elements. Trends in Genetics 15: 326–332. | |
| Reznikoff WS, Bhasin A, Davies DR et al. (1999) Tn5: a molecular window on transposition. Biochemical and Biophysical Research Communications 266: 729–734. | |
| Roth DB and Craig NL (1998) VDJ recombination: a transposase goes to work. Cell 94: 1–20. | |
| Sakai JS, Kleckner N, Yang X and Guhathakurta A (2000) Tn10 transpososome assembly involves a folded intermediate that must be unfolded for target capture and strand transfer. EMBO Journal 19: 776–785. | |
