Transposases and Integrases


Transposases and integrases are the proteins that mediate transposition reactions, i.e. the recombination reaction in which discrete DNA segments move between nonhomologous sites.

Keywords: transposition; recombination

Figure 1.

Cut and paste transposition.

Figure 2.

Donor cleavage.

Figure 3.

Retroviral integration.

Figure 4.

Bacteriophage Mu.

Figure 5.

Phosphoryl transfers in transposition.


Further Reading

Beall EL and Rio DC (1996) Drosphilia IRBP/Ku p70 corresponds to the mutagen‐sensitive mus309 gene and is involved in P‐element excision in vivo. Genes and Development 10: 921–933.

Beall EL and Rio DC (1998) Transposase makes critical contacts with, and is stimulated by, single‐stranded DNA at the P element termini in vitro. EMBO Journal 17: 2122–2136.

Becker HA and Kunze R (1997) Maize Activator transposase has a bipartite DNA binding domain that recognizes subterminal sequences and the terminal inverted repeats. Molecular and General Genetics 254: 219–230.

Capy P, Langin T, Higuet D, Maurer P and Bazin C (1997) Do the integrases of LTR‐retrotransposons and class II element transposases have a common ancestor? Genetica 100: 63–72.

Craig NL (1995) Unity in transposition reactions. Science 270: 253–254.

Craig NL (1997) Target site selection in transposition. Annual Review of Biochemistry 66: 437–474.

Craig NL, Craigie R, Gellert M and Lambowitz A (eds) (in press) Mobile DNA II. Washington, DC: American Society for Microbiology.

Grindley NDF and Leschziner AE (1995) DNA transposition: from a black box to a color monitor. Cell 83: 1063–1066.

Haren L, Ton‐Hoang B and Chandler M (1999) Integrating DNA: transposases and retroviral integrases. Annual Review of Microbiology 53: 245–281.

Hickman AB, Li Y, Mathew SV et al. (2000) Unexpected structural diversity in DNA recombination: the restriction endonuclease connection. Molecular Cell 5: 1025–1034.

Kennedy AK, Hanniford DB and Mizuuchi K (2000) Single active site catalysis of the successive phosphoryl transfer steps by DNA transposases: insights form phosphorothioate stereoselectivity. Cell 101: 295–305.

Klobutcher LA and Herrick G (1997) Developmental genome reorganization in ciliated protozoa: the transposon link. Progress in Nucleic Acid Research and Molecular Biology 56: 1–62.

Lavoie BD, Shaw GS, Millner A and Chaconas G (1996) Anatomy of a flexer DNA complex inside a higher‐order transposition intermediate. Cell 85: 761–771.

Mahillon J and Chandler M (1998) Insertion sequences. Microbiology and Molecular Biology Review 62: 725–774.

Plasterk RH, Izsvak Z and Ivics Z (1999) Resident aliens: the Tc1/mariner superfamily of transposable elements. Trends in Genetics 15: 326–332.

Reznikoff WS, Bhasin A, Davies DR et al. (1999) Tn5: a molecular window on transposition. Biochemical and Biophysical Research Communications 266: 729–734.

Roth DB and Craig NL (1998) VDJ recombination: a transposase goes to work. Cell 94: 1–20.

Sakai JS, Kleckner N, Yang X and Guhathakurta A (2000) Tn10 transpososome assembly involves a folded intermediate that must be unfolded for target capture and strand transfer. EMBO Journal 19: 776–785.

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How to Cite close
Craig, Nancy L(Jun 2001) Transposases and Integrases. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1038/npg.els.0000593]