## Quantification of Metabolic Fluxes

Andreas Karoly Gombert, University of São Paulo, São Paulo, Brazil

Jens Nielsen, Technical University of Denmark, Lyngby, Denmark

Published online: May 2003

DOI: 10.1038/npg.els.0000622

### Abstract

The phenotype of an organism grown under defined environmental conditions may be characterized by the fluxes carried out at
each individual reaction in the organism's metabolic network. Metabolic fluxes are nonmeasurable quantities, but they can
be quantified using a combination of specific measurements and mathematical modelling.

Keywords: metabolic flux analysis; mathematical modelling of metabolism; labelling experiments; metabolic engineering

References

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Further Reading

Christensen B and
Nielsen J
(1999)
Metabolic network analysis: a powerful tool in metabolic engineering.
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66: 209–231.

Gombert AK and
Nielsen J
(2000)
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Current Opinion in Biotechnology
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Szyperski T
(1998)
13C‐NMR, MS and metabolic flux balancing in biotechnology research.
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Wiechert W
(2001)
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Metabolic Engineering
3: 195–206.

Wiechert W,
Möllney M,
Petersen S and
de‐Graaf AA
(2001)
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Metabolic Engineering
3: 265–283.