Kalpana Chakraburtty, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
Published online: April 2001
DOI: 10.1038/npg.els.0000686
Elongation factor 3 (EF-3) is uniquely required by the fungal translational apparatus. Understanding its function may provide new insights into the mechanistic aspect of translation for all systems. The information in turn may lead to the development of potential antifungal drugs.
Keywords: protein synthesis; fungal protein; ribosome; ATP-binding protein; RNA-binding protein
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Figure 1. Representation of the reactions of the elongation cycle. EF, elongation factors; A, P and E represent the binding sites for aminoacyl-, peptidyl- and deacyl-tRNA on the ribosome shown by the corresponding symbols in the diagram. PRE and POST represent the pre- and the posttranslocational states of the ribosome. The reactions of the elongation cycle require guanosine triphosphate (GTP) for EF-1 and EF-2 function, adenosine triphosphate (ATP) for EF-3 function. GDP, guanosine diphosphate; P
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| References | |
| Belfield GP and Tuite MF (1993) Translation elongation factor 3: A fungus-specific translation factor? Molecular Microbiology 9: 411–418. | |
| Belfield GP, Ross-Smith NJ and Tuite MF (1995) Translational elongation factor 3 (EF-3): an evolving eukaryotic ribosomal protein? Journal of Molecular Evolution 41: 376–387. | |
| book Chakraburtty K (1992) "Elongation factor 3 – A unique fungal protein". In: Fernandes PB (ed.) New Approaches to Antifungal Drugs, pp. 114–142. Boston: Birkhauser. | |
| Chakraburtty K and Kamath A (1988) Protein synthesis in yeast. International Journal of Biochemistry 20: 581–590. | |
| Chakraburtty K and Triana-Alonso FJ (1998) Yeast elongation factor 3: structure and function. Biological Chemistry 379: 831–840. | |
| DiDomenico BJ, Lupisella J, Sandbaken M and Chakraburtty K (1992) Isolation and sequence analysis of the gene encoding translation elongation factor 3 from Candida albicans. Yeast 8: 337–352. | |
| Gontarek RR, Li H, Nurse K and Prescott CD (1998) The N-terminus of eukaryotic translation elongation factor 3 interacts with 18S rRNA and 80S ribosomes. Journal of Biological Chemistry 273: 10249–10252. | |
| Hutchison JS, Feinberg B, Rothwell TC and Moldave K (1984) Monoclonal antibody specific for yeast elongation factor 3. Biochemistry 23: 3055–3063. | |
| Kambampati R and Chakraburtty K (1997) Functional subdomains of yeast EF-3: Localization of the ribosomal-binding domain. Journal of Biological Chemistry 272: 6377–6381. | |
| Nierhaus KH (1990) The allosteric three-site model for the ribosomal elongation cycle: Features and future. Biochemistry 29: 4297–4310. | |
| Triana FJ, Chakraburtty K and Nierhaus KH (1995) Role of EF-3 in allosteric interactions of A- and E-sites of eukaryotic ribosomes. Journal of Biological Chemistry 270: 20473–20478. | |
| Further Reading | |
| Bianchet MA, Ko YH, Amzel M and Pedersen PL (1997) Modeling of nucleotide binding domains of ABC transporter proteins based on a Fl-ATPase/recA topology: Structural model of the nucleotide binding domains of cystic fibrosis transmembrane conductance regulator. Journal of Bioenergetics and Biomembranes 29: 503–524. | |
| Chakraburtty K (1999) Functional interaction of elongation factor 3 with yeast ribosomes. International Journal of Biochemistry and Cell Biology 31: 163–173. | |
| book Hershey JWB, Mathews MB and Sonenberg N (eds) (1996) Translational Control. New York: Cold Spring Harbor Laboratory Press. | |
| book Matheson AT, Davies JE, Dennis PP and Hill WE (eds) (1995) Frontiers in Translation, vol. 73. Ottawa, Canada: NRC Research Press. | |
| book Nierhaus KH, Franceschi F, Subramanian AR, Erdmann VA and Wittman-Liebold B (eds) (1993) The Translational Apparatus. New York: Plenum Press. | |
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