Enzyme Activity and Assays


Enzyme activity refers to the general catalytic properties of an enzyme, and enzyme assays are standardized procedures for measuring the amounts of specific enzymes in a sample.

Keywords: enzymes; assays; methods

Figure 1.

Comparison of a conventional Michaelis–Menten enzyme with an allosteric enzyme: the rate variation with substrate concentration.

Figure 2.

Four different ways of assaying the enzyme invertase.


Further Reading

Brooks SPJ and Suelter CH (1989) Review: practical aspects of coupling enzyme theory. Analytical Biochemistry 176: 1–14.

Eisenthal R and Danson MJ (1992) Enzyme Assays: A Practical Approach. Oxford: IRL Press.

Engel PC (ed.) (1996) Enzymology Labfax. Oxford: Bios Scientific.

Price NC and Stevens L (1989) Fundamentals of Enzymes. Oxford: Oxford University Press.

Purich DL (ed.) (1996) Contemporary Enzyme Kinetics and Mechanisms. New York: Academic Press.

Rossomando EF (1990) Measurement of enzyme activity. Methods in Enzymology 182: 38–49.

Scopes RK (1993) Protein Purification, Principles and Practice. New York: Springer‐Verlag.

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Scopes, Robert K(Jul 2002) Enzyme Activity and Assays. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0000712]