Enzyme activity refers to the general catalytic properties of an enzyme, and enzyme assays are standardized procedures for measuring the amounts of specific enzymes in a sample.
Keywords: enzymes; assays; methods
Enzyme Activity and Assays
Robert K Scopes, La Trobe University, Bundoora, Victoria, Australia
Published online: July 2002
DOI: 10.1038/npg.els.0000712
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Figure 1. Comparison of a conventional Michaelis–Menten enzyme with an allosteric enzyme: the rate variation with substrate concentration.
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Figure 2. Four different ways of assaying the enzyme invertase.
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| Further Reading | |
| Brooks SPJ and Suelter CH (1989) Review: practical aspects of coupling enzyme theory. Analytical Biochemistry 176: 1–14. | |
| book Eisenthal R and Danson MJ (1992) Enzyme Assays: A Practical Approach. Oxford: IRL Press. | |
| book Engel PC (ed.) (1996) Enzymology Labfax. Oxford: Bios Scientific. | |
| book Price NC and Stevens L (1989) Fundamentals of Enzymes. Oxford: Oxford University Press. | |
| book Purich DL (ed.) (1996) Contemporary Enzyme Kinetics and Mechanisms. New York: Academic Press. | |
| Rossomando EF (1990) Measurement of enzyme activity. Methods in Enzymology 182: 38–49. | |
| book Scopes RK (1993) Protein Purification, Principles and Practice. New York: Springer-Verlag. | |
