Regulation by Covalent Modification

Abstract

Covalent modifications are enzyme‐catalysed alterations of synthesised proteins and include the addition or removal of chemical groups. Modifications can target a single type of amino acid or multiple amino acids and will change the chemical properties of the site. Consequences on the structure and functions of the protein modified are manifested providing a sensitive method for cellular regulation. The modification of the protein can be reversible or irreversible; in most instances, there are different enzymes responsible for the forward and reverse reactions. Reversible modifications typically associated with the regulation of signalling and metabolic processes and select critical targets can be modified by multiple types of modifications at multiple types of amino acids with multiple number of modifications. Irreversible modifications are more energetically costly requiring the synthesis of new protein before the functional modification can be accomplished if needed.

Key Concepts:

  • Select enzymes catalyse the transfer of a chemical group from a donor to a target protein.

  • The target protein is modified on a specific amino acid consistent with the chemistry of the modifying group.

  • Modifications can influence the properties of the target protein with changes in structure, charge and function.

  • Enzyme‐catalysed modifications can be reversible or irreversible; reversible modifications typically require different enzymes for the reverse reaction.

  • Reversible modifications typically associated with regulatory processes or in regulatory steps of metabolic and signalling pathways.

  • Select critical processes and proteins are targets of multiple types and sites of reversible modifications.

  • Irreversible modifications require the synthesis of protein.

  • Irreversible modifications typically associated with physiological cascade processes such as blood coagulation.

Keywords: co‐translation modification; post‐translational modification

Figure 1.

Generalised scheme for covalent modification reactions. Shown is an example of a transfer of a generic group (X) to a specific acceptor residue (A) on the target protein. The site (A) becomes modified. In other reactions, cleavage for example, a group may be removed from the target site (A).

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How to Cite close
Martin, Bruce L(Aug 2014) Regulation by Covalent Modification. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0000866.pub3]