Antiserum is a general term used to denote the noncellular component of blood (serum) containing a mixture of antibodies (polyclonal), the donor usually having been actively immunized with a known antigen.

Keywords: antigen; antibody; immunization; monoclonal; polyclonal


Catty D and Raykundalia C (1988) Production and quality control of polyclonal antibodies. In: Catty D (ed.) Antibodies, vol. I, A Practical Approach. Oxford: IRL Press.

Galfré G and Milstein C (1981) Preparation of monoclonal antibodies: strategies and procedures. Methods in Enzymology 73: 1–46.

Green JA and Manson MM (1998) Production of polyclonal antisera. In: Pound J (ed.) Immunochemical Protocols, 2nd edn, pp. 1–13. Totowa, NJ: Humana Press.

Köhler G and Milstein C (1975) Continuous culture of fused cells secreting antibody of predefined specificity. Nature 256: 495–497.

Stewart‐Tull DES (1997a) The Choice and Mechanism of Action of Adjuvants. Switzerland: Horwood Academic Publications.

Stewart‐Tull DES (1997b) The use of adjuvants in the production of polyclonal antisera to protein antigens. In: van Zutphen LFM and Balls M (eds) Animal Alternatives, Welfare and Ethics: Developments in Animal and Veterinary Sciences, vol. 27, pp. 989–994. Amsterdam: Elsevier.

Further Reading

Catty D (ed.) (1988) Antibodies, vol. I, A Practical Approach. Oxford: IRL Press.

Catty D (ed.) (1989) Antibodies, vol. II, A Practical Approach. Oxford: IRL Press.

Goding JW (1996) Monoclonal Antibodies: Principles and Practice, 3rd edn. London: Academic Press.

Pound JD (ed.) (1998) Immunochemical Protocols, 2nd edn. Methods in Molecular Biology, vol. 80. Totowa, NJ: Humana Press.

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Goodall, Delia Margaret, and Raykundalia, Chandra(Apr 2001) Antiserum. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1038/npg.els.0000911]