Restriction Enzymes

Abstract

Type II restriction enzymes are the molecular scissors that catalyse the double‐strand cleavage of deoxyribonucleic acid (DNA) at specific base sequences. They are essential tools for manipulating DNA including but not limited to cloning, analysis and sequencing. Recent advances have made it possible to design and engineer chimaeric nucleases to target specific DNA sites within a genome thus making them useful tools to carry out gene therapy.

Keywords: restriction enzymes; endonucleases; methylases; restriction–modification; recombinant DNA; chimaeric nucleases

Figure 1.

Representation of multimodular enzymes. (a) FokI (a type IIs restriction enzyme); (b) I‐TevI (a homing endonuclease). R, recognition domain; EN, endonuclease domain.

Figure 2.

Representation of various chimaeric nucleases. ZF‐EN, Ubx‐EN and Gal4‐EN were made by fusing the isolated nuclease domain (EN) of FokI to the zinc‐finger motif, helix–turn–helix motif and Gal4, respectively. R, recognition domain; EN, endonuclease domain.

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References

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How to Cite close
Mani, Mala, Kandavelou, Karthikeyan, and Chandrasegaran, Srinivasan(Dec 2007) Restriction Enzymes. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0000973.pub3]