Ligation: Theory and Practice
Karthikeyan Kandavelou, Johns Hopkins University, Maryland, USA
Mala Mani, Johns Hopkins University, Maryland, USA
Sekhar PM Reddy, Johns Hopkins University, Baltimore, Maryland, USA
Srinivasan Chandrasegaran, Johns Hopkins University, Baltimore, Maryland, USA
Published online: September 2005
DOI: 10.1038/npg.els.0003838
Abstract
Ligation is the process by which NAD DNA ligases catalyse the formation of phosphodiester bonds between juxtaposed 3′‐hydroxyl
group and 5′‐phosphate termini in duplex deoxyribonucleic acid (DNA). Ligases use adenosine triphosphate or nicotinamide–adenine
dinucleotide (NAD) as cofactors for this covalent joining of DNA.
Keywords: DNA ligases; recombinant DNA; ligase chain reaction; mammalian ligases
Further Reading
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