Ivan Sadowski, University of British Columbia, Vancouver, Canada
Published online: April 2001
DOI: 10.1038/npg.els.0000977
Proteins are composed of multiple functional and structural domains. Using recombinant DNA techniques, it is possible to create chimaeric proteins with novel functions for research or biotechnology purposes, and for the development of novel therapies.
Keywords: recombinant DNA; fusion proteins; protein domains; chimaeric proteins; purification
| Further Reading | |
| Brizzard BL, Chubet RG and Vizard DL (1994) Immunoaffinity purification of FLAG epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution. Biotechniques 16: 730–735. | |
| Chong S, Mersha FB, Comb DG et al. (1997) Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene 192: 271–281. | |
| Dohmen RJ, Wu P and Varshavsky A (1994) Heat-inducible degron: a method for constructing temperature-sensitive mutants. Science 263: 1273–1276. | |
| Farrar MA, Alberol I and Perlmutter RM (1996) Activation of the Raf-1 kinase cascade by coumermycin-induced dimerization. Nature 383: 178–181. | |
| Gossen M, Freundlieb S, Bender G, Muller G, Hillen W and Bujard H (1995) Transcriptional activation by tetracyclines in mammalian cells. Science 268: 1766–1769. | |
| Hayden MS, Gilliland LK and Ledbetter JA (1997) Antibody engineering. Current Opinion in Immunology 9: 201–212. | |
| Kim JS and Raines RT (1993) Ribonuclease S-peptide as a carrier in fusion proteins. Protein Science 2: 348–356. | |
| LaVallie ER, DiBlasio EA, Kovacic S, Grant KL, Schendel PF and McCoy JM (1993) A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm. Biotechnology 11: 187–193. | |
| Lin A, Minden A, Martinetto H et al. (1995) Identification of a dual specificity kinase that activates the Jun kinases and p38-Mpk2. Science 268: 286–290. | |
| Maina CV, Riggs PD, Grandea AGd et al. (1988) An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein. Gene 74: 365–373. | |
| Nilsson J, Larsson M, Stahl S, Nygren PA and Uhlen M (1996) Multiple affinity domains for the detection, purification and immobilization of recombinant proteins. Journal of Molecular Recognition 9: 585–594. | |
| Pawson T (1995) Protein modules and signalling networks. Nature 373: 573–580. | |
| Pengue G, Caputo A, Rossi C, Barbanti-Brodano G and Lania L (1995) Transcriptional silencing of human immunodeficiency virus type 1 long terminal repeat-driven gene expression by the Kruppel-associated box repressor domain targeted to the transactivating response element. Journal of Virology 69: 6577–6580. | |
| Picard D (1993) Steroid-binding domains for regulating the functions of heterologous proteins in cis. Trends in Cell Biology 3: 278–280. | |
| Sadowski I (1995) Uses for GAL4 expression in mammalian cells. Genetic Engineering 17: 119–148. | |
| Sonnhamer ELL and Kahn D (1994) Modular arrangement of proteins as inferred from analysis of homology. Protein Science 3: 482–492. | |
| Woodworth TG and Nichols JC (1993) Recombinant fusion toxins – a new class of targeted biologic therapeutics. Cancer Treatment and Research 68: 145–160. | |
| Zheng CF, Simcox T, Xu L and Vaillancourt P (1997) A new expression vector for high level protein production, one step purification and direct isotopic labeling of calmodulin-binding peptide fusion proteins. Gene 186: 55–60. | |
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