Genetic Engineering: Reporter Genes

Reporter genes are easily measured surrogates for a molecule of interest.

Keywords: counterselection; fluorescence; screening; selection

 References
    Clarke DJ, Gimenez-Abian JF, Tonnies H et al. (1998) Creation of monosomic derivatives of human cultured cell lines. Proceedings of the National Academy of Sciences of the USA 95: 167–171.
    Cost GJ and Boeke JD (1996) A useful colony colour phenotype associated with the yeast selectable/counter-selectable marker MET15. Yeast 12: 939–941.
    Hieter P, Pridmore D, Hegemann JH et al. (1985) Functional selection and analysis of yeast centromeric DNA. Cell 42: 913–921.
    Lawes M and Maloy S (1995) MudSacI, a transposon with strong selectable and counterselectable markers: use for rapid mapping of chromosomal mutations in Salmonella. Journal of Bacteriology 177: 1383–1387.
    Nirenberg S and Cepko C (1993) Targeted ablation of diverse cell classes in the nervous system in vivo. Journal of Neuroscience 13: 3238–3251.
    Nolan GP, Fiering S, Nicolas JF and Herzenberg LA (1988) Fluorescence-activated cell analysis and sorting of viable mammalian cells based on beta-d-galactosidase activity after transduction of E. coli lacZ. Proceedings of the National Academy of Sciences of the USA 85: 2603–2607.
    Ntziachristos V (2006) Fluorescence molecular imaging. Annual review of Biomedical Engineering 8: 1–33.
    Salmon MA, Van Melderen L, Bernard P and Couturier M (1994) The antidote and autoregulatory functions of the F plasmid CcdA protein: a genetic and biochemical survey. Molecular and General Genetics 244: 530–538.
    Serganova I and Blasberg R (2005) Reporter gene imaging: potential impact on therapy. Nuclear Medicine and Biology 32: 763–780.
    Tjuvajev JG, Stockhammer G, Desai R et al. (1995) Imaging the expression of transfected genes in vivo. Cancer Research 55: 6126–6132.
    Walbot V, de Wet JR and Fromm M (1987) Visual assays of transformation in plant cells. Basic Life Sciences 41: 183–188.
    Zlokarnik G, Negulescu PA, Knapp TE et al. (1998) Quantitation of transcription and clonal selection of single living cells with beta-lactamase as reporter. Science 279: 84–88.
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Lawler, Joseph F, Hyland, Edel M, and Boeke, Jef D(Jul 2008) Genetic Engineering: Reporter Genes. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0000980.pub2]