Phage Display Technologies

Abstract

Phage display is a method that uses bacterial virus (phage) as a vehicle to express diverse protein or peptide sequences as part of the phage coat protein by cloning deoxyribonucleic acid (DNA) fragments in frame with phage coat protein genes. Upon viral infection, the expressed peptides are displayed on the surface of the viral particle. Depending on the DNA fragments cloned, phage display allows for expression of either short peptides or large proteins, such as immunoglobulin, on the viral surface.

Keywords: phage; protein; library; antibody; plasmid

Figure 1.

A schematic diagram showing (a) wild type filamentous phage; (b) pIII‐based phage display; (c) pVIII‐based phage display.

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References

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How to Cite close
Stricker, Nicole, and Li, Min(Apr 2001) Phage Display Technologies. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0000982]