Phage Display Technologies

Nicole Stricker, Johns Hopkins University, Baltimore, Maryland, USA
Min Li, Johns Hopkins University, Baltimore, Maryland, USA

Published online: April 2001

DOI: 10.1038/npg.els.0000982

Phage display is a method that uses bacterial virus (phage) as a vehicle to express diverse protein or peptide sequences as part of the phage coat protein by cloning deoxyribonucleic acid (DNA) fragments in frame with phage coat protein genes. Upon viral infection, the expressed peptides are displayed on the surface of the viral particle. Depending on the DNA fragments cloned, phage display allows for expression of either short peptides or large proteins, such as immunoglobulin, on the viral surface.

Keywords: phage; protein; library; antibody; plasmid

Figure 1. A schematic diagram showing (a) wild type filamentous phage; (b) pIII-based phage display; (c) pVIII-based phage display.
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 References
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Related Sub-Topics:

DNA Viruses | Virus Structure | Virus Culture | Translation and Protein synthesis | Virus Structure and Function | Sample Preparation in Structural Biology | Techniques and Tools in Molecular Biology
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Article Title: Phage Display Technologies
 
How to Cite close
Stricker, Nicole, and Li, Min(Apr 2001) Phage Display Technologies. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0000982]