Protein Production for Biotechnology

Sarah E Giuliani, Argonne National Laboratory, Argonne, IL, USA
Elizabeth V Landorf, Argonne National Laboratory, Argonne, IL, USA
Frank R Collart, Argonne National Laboratory, Argonne, IL, USA

Published online: September 2007

DOI: 10.1002/9780470015902.a0000983.pub2

Purified proteins are a starting point for many experimental, therapeutic and commercial applications. Developments in biotechnology have generated a collection of resources to enable production of proteins from a variety of sources.

Keywords: protein expression; fusion tag; cell-free

 Further Reading
    Dawson PE and Kent SB (2000) Synthesis of native proteins by chemical ligation. Annual Review of Biochemistry 69: 923–960.
    Graumann K and Premstaller A (2006) Manufacturing of recombinant therapeutic proteins in microbial systems. Biotechnology Journal 1(2): 164–186.
    Houdebine LM (2000) Transgenic animal bioreactors. Transgenic Research 9(4–5): 305–320.
    Katzen F, Chang G, Kudlicki W et al. (2005) The past, present and future of cell-free protein synthesis. Trends in Biotechnology 23(3): 150–156.
    Kost TA, Condreay JP, Jarvis DL et al. (2005) Baculovirus as versatile vectors for protein expression in insect and mammalian cells. Nature Biotechnology 23(5): 567–575.
    Malissard M, Zeng S, Berger EG et al. (1999) The yeast expression system for recombinant glycosyltransferases. Glycoconjugate Journal 16(2): 125–139.
    Terpe K (2006) Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems. Applied Microbiolology and Biotechnology 72(2): 211–222.
    Thomas P and Smart TG (2005) HEK293 cell line: a vehicle for the expression of recombinant proteins. Journal of Pharmacological and Toxicological Methods 51(3): 187–200.
    Vitale A and Pedrazzini E (2005) Recombinant pharmaceuticals from plants: the plant endomembrane system as bioreactor. Molecular Interventions 5(4): 216–225.
    Yin J, Li G, Ren X et al. (2007) Select what you need: a comparative evaluation of the advantages and limitations of frequently used expression systems for foreign genes. Journal of Biotechnology 127(3): 335–347.

Related Sub-Topics:

Translation and Protein synthesis | Protein Folding, Evolution and Degradation | Sample Preparation in Structural Biology | Techniques and Tools in Molecular Biology
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Article Title: Protein Production for Biotechnology
 
How to Cite close
Giuliani, Sarah E, Landorf, Elizabeth V, and Collart, Frank R(Sep 2007) Protein Production for Biotechnology. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0000983.pub2]