Transfection of DNA into Mammalian Cells in Culture

Abstract

Transfection is the transfer of DNA into cells and is a key technique for analysing mammalian cells. A variety of methods are employed to achieve transfection for use in genetic studies.

Keywords: liposome; electroporation; transient transfection; stable transfection; extrachromosomal vector; integrase; reporter gene; transgene; selectable marker

References

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Further Reading

Hug P and Sleight RG (1991) Liposomes for the transformation of eukaryotic cells. Biochimica et Biophysica Acta 1097: 1–17.

Kingston RE (1997) Introduction of DNA into mammalian cells. In: Ausubel FM, Brent R, Kingston RE et al. (eds) Current Protocols in Molecular Biology, pp. 9.0–9.7. Boston, MA: John Wiley & Sons

Sambrook J, Fritsch EF and Maniatis T (1989) Expression of cloned genes in cultured mammalian cells. In: Molecular Cloning: A Laboratory Manual, chap 16. Cold Spring Harbor NY: Cold Spring Harbor Laboratory Press.

Tsien R (1998) The green fluorescent protein. Annual Review of Biochemistry 67: 509–544.

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Chalberg, Thomas W, Phillips, Julie E, and Calos, Michele P(Sep 2005) Transfection of DNA into Mammalian Cells in Culture. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003928]