Transfection of DNA into Mammalian Cells in Culture

Thomas W Chalberg, Stanford University, California, USA
Julie E Phillips, Stanford University, California, USA
Michele P Calos, Stanford University, California, USA

Published online: September 2005

DOI: 10.1038/npg.els.0003928

Transfection is the transfer of DNA into cells and is a key technique for analysing mammalian cells. A variety of methods are employed to achieve transfection for use in genetic studies.

Keywords: liposome; electroporation; transient transfection; stable transfection; extrachromosomal vector; integrase; reporter gene; transgene; selectable marker

 References
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 Further Reading
    Hug P and Sleight RG (1991) Liposomes for the transformation of eukaryotic cells. Biochimica et Biophysica Acta 1097: 1–17.
    book Kingston RE (1997) "Introduction of DNA into mammalian cells". In: Ausubel FM, Brent R, Kingston RE et al. (eds) Current Protocols in Molecular Biology, pp. 9.0–9.7. Boston, MA: John Wiley & Sons
    book Sambrook J, Fritsch EF and Maniatis T (1989) Expression of cloned genes in cultured mammalian cells. In: Molecular Cloning: A Laboratory Manual, chap 16. Cold Spring Harbor NY: Cold Spring Harbor Laboratory Press.
    Tsien R (1998) The green fluorescent protein. Annual Review of Biochemistry 67: 509–544.

Related Sub-Topics:

Sample Preparation in Structural Biology | Techniques and Tools in Clinical Genetics | Gene Therapy | Techniques and Tools in Molecular Biology | Nonchromosomal DNA
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Article Title: Transfection of DNA into Mammalian Cells in Culture
 
How to Cite close
Chalberg, Thomas W, Phillips, Julie E, and Calos, Michele P(Sep 2005) Transfection of DNA into Mammalian Cells in Culture. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003928]