| References |
|
|
Antharavally BS,
Carter B,
Bell PA and
Krishna MA
(2004)
A high-affinity reversible protein stain for Western blots.
Analytical Biochemisty
329:
276280.
|
|
|
Banks RE,
Craven RA,
Harnden PA and
Selby PJ
(2003)
Use of a sensitive EnVision+-based detection system for Western blotting: avoidance of streptavidin binding to endogenous biotin and biotin-containing proteins in kidney and other tissues.
Proteomics
3:
558561.
|
|
|
book
Bjerrum OJ,
Larsen KP and
Heegaard NHH
(1988)
"Specificity problems and troubleshooting with an atlas of immunoblotting artifacts".
In: Bjerrum OJ and
Heegaard NHH (eds.)
Handbook of Immunoblotting of Proteins, vol. I: Technical Descriptions,
pp. 227254.
Boca Raton, FL: CRC Press.
|
|
|
Bjerrum OJ,
Selmer JC and
Lihme A
(1987)
Native immunoblotting: transfer of membrane proteins in the presence of non-ionic detergent.
Electrophoresis
8:
388397.
|
|
|
Choi J-Y,
Park H-J,
Seong Y-M et al.
(2005)
Fine epitope mapping of monoclonal antibodies specific to human synuclein.
Neuroscience Letters
397:
5358.
|
|
|
Daban JR
(2001)
Fluorescent labeling of proteins with nile red and 2-methoxy-2,4-diphenyl-3(2H)-furanone: physicochemical basis and application to the rapid staining of sodium dodecyl sulfate polyacrylamide gels and Western blots.
Electrophoresis
22:
874880.
|
|
|
Dakessio J and
Ashley R
(1992)
Highly sensitive enhanced chemiluminescence immunodetection method for herpes simplex virus type 2 Western immunoblot.
Journal of Clinical Microbiology
30:
10051007.
|
|
|
De Lemos EA,
Beém ZR,
Santos A and
Ferreira AW
(2007)
Characterization of the Western blotting IgG reactivity patterns in the clinical phases of acquired syphilis.
Diagnostic Mircrobiology and Infectious Disease
58:
177183.
|
|
|
book
Dunbar BS (ed.)
(1994)
Protein Blotting: A Practical Approach.
Oxford: Oxford University Press.
|
|
|
Dunn MJ
(1999)
Detections of total proteins on western blots of 2-D polyacrylamide gel.
Methods Molecular Biology
112:
319329.
|
|
|
Durrant I
(1990)
Light based detection of biomolecules.
Nature
346:
297298.
|
|
|
other
Edmondson DG and
Roth SY
(2001) Identification of protein interactions by far Western analysis. Current Protocol in Molecular Biology Chap. 17: Unit 17.2; Chap. 20: Unit 20.6.
|
|
|
Egger D and
Bienz K
(1994)
Protein (Western) blotting.
Molecular Biotechnology
1:
289305.
|
|
|
Eynard L and
Laurière M
(1998)
The combination of Indian ink staining with immunochemiluminescence detection allows precise identification of antigens on blots: application to the study of glycosylated barley storage proteins.
Electrophoresis
19:
13941396.
|
|
|
book
Gershoni JM
(1988)
"Protein blotting: a manual".
In: Glick D (ed.)
Methods of Biochemical Analysis,
pp. 158.
New York: Wiley.
|
|
|
González-Buitrago JM
(2006)
Multiplexed testing in the autoimmunity laboratory.
Clinical Chemistry and Laboratory Medicine
44(10):
11691174.
|
|
|
Hall RA
(2004)
Studying proteinprotein interactions via blot overlay or Far Western blot.
Methods of Molecular Biology
261:
167174.
|
|
|
book
Heegaard NHH and
Bjerrum OJ
(1988)
"Immunoblotting general principles and procedures".
In: Bjerrum OJ and
Heegaard NHH (eds)
Handbook of Immunoblotting of Proteins. Vol. I. Technical Descriptions,
pp. 125.
Boca Raton, FL: CRC Press.
|
|
|
Houen G,
Bruun L and
Barkholt V
(1997)
Combined immunostaining and Coomassie Brilliant Blue staining of polyvinylidene difluoride membranes without organic solvent.
Electrophoresis
18:
701705.
|
|
|
Howell JM,
Winstone TL,
Coorssen JR and
Turner RJ
(2006)
An evaluation of in vitro proteinprotein interaction techniques: assessing contaminating background proteins.
Proteomics
6:
20502069.
|
|
|
Kaufmann SH,
Ewing M and
Shaper JH
(2000)
The erasable blot.
Analytical Biochemistry
161:
8995.
|
|
|
Kittler JM,
Meisler NT,
Viceps-Madora D,
Cidlowski JA and
Thanassi JW
(1984)
A general immunochemical method for detecting proteins on blots.
Analytical Biochemistry
137:
210216.
|
|
|
Knudsen KA
(1985)
Proteins transferred to nitrocellulose for use as immunogens.
Analytical Biochemistry
147:
285288.
|
|
|
Konse T,
Takahashi T,
Nagashima H and
Iwaoka T
(1993)
Blotting membrane micropreparation in capillary electrophoresis to evaluate enzyme purity and activity.
Analytical Biochemistry
214:
179181.
|
|
|
Koticha D,
Mabuchi M and
Carelli M
(2006)
Transfer membrane improves fluorescent detection of proteins.
Bioscience Technology
31:
1819.
|
|
|
LeGendre N
(1990)
Immobilon-P transfer membrane: applications and utility in protein biochemical analysis.
Biotechniques
9:
788804.
|
|
|
Lewis SA,
Grimshaw KEC,
Warner JO and
Hourhane Jo'B
(2005)
The promiscuity of immunoglobulin E binding to peanut allergens, as determined by Western blotting, correlates with the severity of clinical symptoms.
Clinical Experimental Allergy
35:
767773.
|
|
|
Martin K,
Hart C,
Liu J,
Leung WY and
Patton WF
(2003)
Simultaneous trichromatic fluorescence detection of proteins on Western blots using an amine-reactive dye in combination with alkaline phosphatase- and horseradish peroxidase-antibody conjugates.
Proteomics
3(7):
12151227.
|
|
|
book
Matsudaira P (ed.)
(1993)
A Practical Guide to Protein and Peptide Purification for Microsequencing.
San Diego, CA: Academic Press.
|
|
|
McDonald K,
Elbaggan A and
Pekelis M
(2006)
Fluorescent nanoparticles for Western blotting.
Bio-rad Bulletin
3179:
16,
www.Bio-rad.com.
|
|
|
Olesen CE,
Yan YX,
Liu B et al.
(2000)
Novel methods for chemiluminescent detection of reporter enzymes.
Methods of Enzymology
326:
175202.
|
|
|
Patel S,
McLauchlin J and
Casemore DP
(1997)
A simple SDS-PAGE immunoblotting technique using an enhanced chemiluminescence detection system to identify polyclonal antibody responses to complex cryptosporidial antigen preparations following a monoclonal antibody retest and image overlay technique.
Journal of Immunological Methods
205:
157161.
|
|
|
Pemawansa KPW,
Heisler MD,
Blackwell SL et al.
(1990)
An advanced affinity membrane for covalent binding of amino ligands.
Biotechniques
9:
352356.
|
|
|
Qiu J,
Madoz-Gurpide J,
Misek DE et al.
(2004)
Development of natural protein microarrays for diagnosing cancer based on an antibody response to tumor antigens.
Journal of Proteome Research
3(2):
261267.
|
|
|
Schechtman D,
Murriel C,
Bright R and
Mochly Rosen D
(2003)
Overlay method for detecting proteinprotein interactions.
Methods of Molecular Biology
233:
351357.
|
|
|
Singer D,
Herth N,
Kuhlmann J et al.
(2008)
Mapping of phosphorylation-dependent anti-tau monoclonal antibodies in immunoblots using human tau-constructs synthesized by native chemical ligation.
Biochemical and Biophysical Research Communications
367(2):
318322. Epub 26 December 2007.
|
|
|
Sorensen BK,
Hojrup P,
Ostergard E et al.
(2002)
Silver staining of proteins on electroblotting membranes and intensification of silver staining of proteins separated by polyacrylamide gel electrophoresis.
Analytical Biochemistry
304:
3341.
|
|
|
Stott DI
(1989)
Immunoblotting and dot blotting.
Journal of Immunological Methods
119:
153187.
|
|
|
Sulimenko T and
Draber P
(2004)
A fast and simple dot-immunobinding assay for quantification of mouse immunoglobulins in hybridoma culture supernatants.
Journal of Immunological Methods
289:
8995.
|
|
|
Taki T,
Handa S and
Ishikawa D
(1994)
Blotting of glycolipids and phospholipids from a high-performance thin-layer chromatogram to a polyvinylidene difluoride membane.
Analytical Chemistry
221:
312316.
|
|
|
Tamby MC,
Servettaz A,
Guilpan P et al.
(2007)
Immunoblotting on Hep-2 cells increases the detection of antitopoisomerase 1 antibodies in patients with systemic sclerosis.
Clinical Immunology
123:
8288.
|
|
|
Tarlton JF and
Knight PJ
(1996)
Comparison of reflectance and transmission densitometry, using document and laser scanners, for quantitation of stained Western blots.
Analytical Biochemistry
237:
123128.
|
|
|
Top KP,
Hatleberg G,
Berggren KN et al.
(2001)
Green/red dual fluorescence detection of total protein and alkaline phosphate-conjugated probes on blotting membranes.
Electrophoresis
22:
896905.
|
|
|
book
Ursitti JA,
Mozdzanowski J and
Speicher DW
(1995)
"Electroblotting from polyacrylamide gels".
In: Coligan JE,
Dunn BM,
Ploegh HL,
Speicher DW and
Wingfield PT (eds)
Current Protocols in Protein Science,
pp. 10.7.110.7.14.
New York: Wiley.
|
|
|
Vianna COA,
Júnior S and
Martins MGT
(2006)
Evaluation of murine monoclonal antibodies targeting different epitopes of the hepatitis B virus surface antigen by using immunological as well as molecular biology and biochemical approaches.
Journal of Immunological Methods
313:
3847.
|
|
|
Vierck JL,
Bryne KM and
Dodson MV
(2000)
Evaluating dot and Western blots using image analysis and pixel quantification of electronic images.
Methods of Cell Science
22:
313318.
|
|
|
Weghofer M,
Thomas WR,
Pittner G et al.
(2005)
Comparison of purified Dermatophagoides pteronyssinus allergens and extracts by two-dimensional immunoblotting and quantitative immunoglobulin E inhibitors.
Clinical Experimental Allergy
35:
13841391.
|
|
|
Wu Y,
Li Q and
Chen XZ
(2007)
Detecting proteinprotein interactions by Far western blotting.
Nature Protocols
2:
32783284.
|
| Further Reading |
|
|
book
Gallagher S,
Winston SE,
Fuller SA and
Hurrell JGR
(1998)
"Immunoblotting and immunodetection".
In: Coligan JE,
Kruisbeek AM,
Margulies DH,
Shevach EM and
Strober W (eds)
Current Protocols in Immunology,
pp. 8.10.18.10.21.
New York: Wiley.
|
|
|
Kurien BT and
Scofield RH
(2003)
Protein blotting: a review.
Journal of Immunological Methods
274:
115.
|
|
|
Stott DI
(2000)
Immunoblotting, dot-blotting, and ELISPOT assays: methods and applications.
Journal of Immunoassay
21:
273296.
|