Prokaryotic Replication Origins: Structure and Function in the Initiation of DNA Replication

Abstract

Replication origins are cis‐acting sequences of a few hundred base pairs required for the replication of adjacent DNA. Their function is recognition of the correct start site and assembly of replication forks at this site.

Keywords: DnaA; initiator protein; initiation of replication; oriC; replication origin

Figure 1.

Map location and structure of the Escherichia coli replication origin, oriC. Triangles indicate promoters. FIS, factor for inversion stimulation; IHF, integration host factor.

Figure 2.

Stages in the initiation of replication at oriC of Escherichia coli. ADP, adenosine diphosphate; ATP, adenosine triphosphate; Pol, polymerase.

Figure 3.

Structures of some bacterial replication origins.

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References

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Further Reading

Del Solar G, Giraldo R, Ruiz‐Echevarría MJ, Espinosa M and Díaz‐Orejas R (1998) Replication and control of circular bacterial plasmids. Microbiology and Molecular Biology Reviews 62: 434–464.

Helinski DR, Toukdarian AE and Novick RP (1996) Replication control and other stable maintenance mechanisms of plasmids. In: Neidhardt FC, Curtiss R III, Ingraham JL et al. (eds) Escherichia coli and Salmonella, Cellular and Molecular Biology, pp. 2295–2324. Washington, DC: ASM Press.

Helmstetter CE (1996) Timing of synthetic activities in the cell cycle. In: Neidhardt FC, Curtiss R III, Ingraham JL et al. (eds) Escherichia coli and Salmonella, Cellular and Molecular Biology, pp 1627–1639 Washington, DC: ASM Press.

Kaguni JM (1997) Escherichia coli DnaA protein: the replication initiator. Molecules and Cells 7: 145–157.

Kornberg A and Baker TA (1992) DNA Replication. New York: Freeman.

Marians KJ (1992) Prokaryotic DNA replication. Annual Review of Biochemistry 61: 673–719.

Messer W (2002) The bacterial initiator DnaA. DnaA and oriC, the bacterial mode to initiate DNA replication. FEMS Microbiology Reviews 26: 355–374.

Messer W and Weigel C (1996) Initation of chromosome replication. In: Neidhardt FC, Curtiss R III and Ingraham J et al. (eds) Escherichia coli and Salmonella, Cellular and Molecular Biology, pp. 1579–1601. Washington, DC: ASM Press.

Skarstad K and Boye E (1994) The initiator protein DnaA: evolution, properties and function. Biochimica et Biophysica Acta 1217: 111–130.

Yoshikawa H and Wake RG (1993) Initiation and termination of chromosome replication. In: Sonenshein AL, Hoch JA and Losick R (eds) Bacillus subtilis and Other Gram‐Positive Bacteria: Biochemistry, Physiology, and Molecular Genetics, pp. 507–528. Washington, DC: ASM Press.

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How to Cite close
Messer, Walter(May 2005) Prokaryotic Replication Origins: Structure and Function in the Initiation of DNA Replication. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0003900]