Viral Culture Methodologies

Abstract

Virus culture is used both to prepare and to analyse viruses. The techniques of virus culture are based on the culture of cells from host organisms, in which the virus is grown and maintained. Viruses may be prepared as components for vaccines or testing kits, or for use as genetic vectors. Cultured viruses may be used to test new antiviral drugs, to increase understanding of cellular biology, or to study the viruses themselves.

Keywords: virus; viral; cell; culture

Figure 1.

Plaque formation by Herpes simplex virus in Vero monkey kidney cells stained with a vital stain (crystal violet). Plaques formed by destruction of the monolayer are the clear areas in the infected wells. Figure kindly provided by Miss S. Argent, Department of Virology, St. Bartholomew's and the Royal London School of Medicine and Dentistry, London.

Figure 2.

Cytopathic effect. (a) Uninfected human embryo lung (HEL) cells and (b) HEL cells infected with Herpes simplex virus (HSV), showing rounding of cells. (c) Uninfected Vero monkey kidney cells and (d) Vero cells infected with HSV virus, showing destruction of cell monolayer (as seen in Figure 1). Figure kindly provided by Miss S. Argent, Department of Virology, St. Bartholomew's and the Royal London School of Medicine and Dentistry, London.

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References

Blake K (1993) Cell culture. In: Harper DR (ed.) Virology Labfax, pp. 51–79. Oxford: Bios Scientific.

Blake K and O'Connell S (1993) Virus culture. In: Harper DR (ed.) Virology Labfax, pp. 81–122. Oxford: Bios Scientific.

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Further Reading

Harper DR (1993) Virology Labfax. Oxford: Bios Scientific.

Kinchington D and Schinazi RF (2000) Antiviral Methods and Protocols. Totowa, NJ: Humana Press.

Lennette EH and Smith TF (1999) Laboratory Diagnosis of Viral Infections. New York: Marcel Dekker.

Mahy BW and Kangro HO (1996) Virology Methods Manual. San Diego: Academic Press.

Schmidt NJ (1995) Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections. Washington DC: American Public Health Association.

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How to Cite close
Harper, David R(Apr 2001) Viral Culture Methodologies. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0001090]