Affinity Chromatography
Giorgio Fassina, TECNOGEN S.C.p.A., Piana di Monte Verna, Italy
Published online: April 2001
DOI: 10.1038/npg.els.0001111
Abstract
Affinity chromatography is a separation technique based on the use of specific and selective immobilized ligands able to associate
reversibly to a desired biomolecule. Samples are passed through affinity columns that are equilibrated with a buffer favouring
interaction between the immobilized ligand and the target biomolecule. Unrelated contaminants will be eluted in the column
void volume and a suitable buffer change will lead to elution of the adsorbed biomolecule.
Keywords: molecular recognition; purification; immobilized ligands; derivatization
References
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Further Reading
Dean PDG,
Johnson WS and
Middle FA
(1984)
Affinity Chromatography: A Practical Approach.
Oxford: IRL Press.
Fassina G and
Chaiken IM
(1987)
Analytical high‐performance affinity chromatography.
Advances in Chromatography
27: 247–297.
Hermanson GT,
Krishna Mallia A and
Smith PK
(1992)
Immobilized Affinity Ligand Techniques.
Orlando: Academic Press.
