Paz Martínez, Universitat Autònoma de Barcelona, Barcelona, Spain
Antoni Iborra, Universitat Autònoma de Barcelona, Barcelona, Spain
Published online: January 2006
DOI: 10.1038/npg.els.0001115
Several strategies are used to provide immortalized monoclonal antibody-secreting B cells, and antibodies by recombinant deoxyribonucleic acid (DNA) technology. Monoclonal antibodies are good specific reagents to recognize any chemical structure. High concentrations of monoclonal antibodies can be obtained and used in diagnostics and human therapy.
Keywords: specific antibodies; monoclonal antibody technology; hybridomas; B-cell diversity; B-cell immortalization
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Figure 1. Generation of monoclonal antibody secretory hybridomas by B-cell fusion. (a) In vivo selection of high-affinity B cells in germinal centres of lymph nodes. Differentiation in plasma B cells secreting high-affinity antibodies. (b) In vitro production of large concentrations of monoclonal antibodies.
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Figure 2. Strategies for human monoclonal antibody production.
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| References | |
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| Further Reading | |
| book Goldsby RA, Kindt TJ, Osborne BA and Kuby J (2003) Immunology 10th edn. New York: W. H. Freeman and Company. | |
| book Paul WE (2003) Fundamental Immunology, 5th edn. Philadelphia, PA: Lippincott, Williams & Wilkins. | |
| book Roitt IM and Delves PJ (2001) Essential Immunology, 10th edn. Oxford: Blackwell Science. Special issue: 25 years of monoclonal antibodies (2000). Immunology Today 21(8): 355–412. | |
| book Zola H (2000) Monoclonal Antibodies. Oxford, UK: BIOS Scientific Publishers Ltd. | |
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