Immunoprecipitation Techniques


Immunoprecipitations are based on two types of interaction: between antigen and antibody, and between antibody and an immunoadsorbent. The antigen–antibody complex will be bound to an insoluble matrix that can be pelleted and removed from the bulk protein. Immunoprecipitation allows rapid and efficient purification of proteins in a native and biochemically active form.

Keywords: antibody; antigen; epitope; immunoprecipitation; protein A

Figure 1.

Flow diagram of general procedure.



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Scheidtmann, Karl‐Heinz(Apr 2001) Immunoprecipitation Techniques. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1038/npg.els.0001180]