Genome Inserts

Abstract

Genome inserts are DNAs, exclusive of duplications, that occur between preexisting genome sequences. They can be detected by their physical features, including the sequence motifs they contain and the size changes they introduce, or by their genetic effects. While experimental genome inserts have an important role in genome analysis, natural genome inserts participate in evolution.

Keywords: integrated genome mapping; euchromosome; prophages; pathogenicity islands; evolution

Figure 1.

Large‐scale comparative genome analysis. (a) Genetic clamping between copies of the Escherichia coli genome. NotI restriction digestion patterns are shown of wild‐type (wt) and mutagenized E. coli genome copies from a nonpathogenic (NP) and a pathogenic (P) strain background. At different genomic locations (at positions 72 min and 78 min), the changes introduced into each strain background by the identical genome insert (crossed between strains by P1 transduction) carrying the NotI recognition site are indicated by missing native NotI fragments and novel NotI subfragments. (b) Heteroduplex analysis of the lambdoid phages genome.

close

Further Reading

Anderson NG (1970) Evolutionary significance of virus infection. Nature 227: 1346–1347.

Campbell A (1981) Evolutionary significance of accessory DNA elements in bacteria. Annual Review of Microbiology 35: 55–83.

Groisman E and Ochman H (1996) Pathogenicity islands: bacterial evolution in quantum leaps. Cell 87: 791–794.

Contact Editor close
Submit a note to the editor about this article by filling in the form below.

* Required Field

How to Cite close
Bloch, Craig A(Apr 2001) Genome Inserts. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0001489]