Plant Cell Walls


Common to all plant species, the cell wall is the tough outer coat that protects the plant cell. The cell wall is mostly carbohydrate‐based, comprising three major classes of polysaccharides: cellulose, hemicellulose and pectin. There are also important structural proteins as well as phenolic and aliphatic polymers. The cell wall provides mechanical strength to the plant body, allowing upright growth and structure formation, and also plays important roles in cellular processes such as cell expansion, tissue differentiation, intercellular communication, water movement and defence responses against pests or pathogens. Cell walls may even be involved in signal sensing during pattern formation in plant development.

Key Concepts

  • The cell wall is the outermost layer of the plant cell.
  • The cell wall consists essentially of three major types of carbohydrates – cellulose, hemicellulose and pectins – and proteins.
  • Some specialised cell wall types, for example woody cell walls also contain phenolic and aliphatic polymers in addition to carbohydrate polymers.
  • The cell wall is a dynamic structure whose composition changes during cell, tissue and plant development and in response to various stresses.
  • The cell wall provides mechanical strength to the plant body.
  • The cell wall plays important roles in processes such as cell expansion, tissue differentiation, intercellular communication, water movement and defence responses against pests or pathogens.

Keywords: cell wall; plant; polysaccharide; structure; function; cell surface; extracellular matrices

Figure 1. Model showing the arrangement of cellulose microfibrils in the walls of a plant cell. Microfibrils are inextensible, so this spiral‐like arrangement dictates that the cylindrical cell will expand predominantly in one dimension, as shown. Many of the cells in an elongating stem or root conform to this model.
Figure 2. Schematic models for pectic polymers. (a) Homogalacturonan can be extensively methyl‐esterified and acetylated. (b) Rhamnogalacturonan I has long arabinan and arabinogalactan side branches, which can be interlinked. (c) Rhamnogalacturonan II has highly complex side chains which show some limited variability between plant species.
Figure 3. Schematic structural models for xyloglucan (a) and xylan (b).
Figure 4. Mechanism of wall loosening caused by the activity of xyloglucan endo‐transglycosylase (XET).
Figure 5. Immunogold localisation of lignin in the secondary cell walls of poplar wood fibre cells. The image shows a cross section of a fibre cell wall which has been treated with a preparation of gold particles coupled with antibodies that recognise lignin. The gold particles are visible as black dots in the S1 and S2 layers of the secondary cell wall, indicating the presence of lignin there, but are absent from the primary wall and middle lamella between two neighbouring cells. Bar is 0.5 µm. Reproduced from Joseleau et al. © Springer.


Albenne C, Canut H, Hoffmann L and Jamet E (2014) Plant cell wall proteins: a large body of data, but what about runaways? Proteomes 2: 224–242.

Barros J, Serk H, Granlund I and Pesquet E (2015) The cell biology of lignification in higher plants. Annals of Botany 115: 1053–1074.

Bonawitz ND and Chapple C (2010) The genetics of lignin biosynthesis: connecting genotype to phenotype. Annual Review of Genetics 44: 337–363.

Bromley JR, Busse‐Wicher M, Tryfona T, et al. (2013) GUX1 and GUX2 glucuronyltransferases decorate distinct domains of glucuronoxylan with different substitution patterns. The Plant Journal 74: 423–434.

Buckeridge MS (2010) Seed cell wall storage polysaccharides: models to understand cell wall biosynthesis and degradation. Plant Physiology 154: 1017–1023.

Canut H, Albenne C and Jamet E (2016) Post‐translational modifications of plant cell wall proteins and peptides: a survey from a proteomics point of view. Biochimica et Biophysica Acta 1864: 983–990.

Carpita NC (2011) Update on mechanisms of plant cell wall biosynthesis: how plants make cellulose and other (1→4)‐β‐d‐glycans. Plant Physiology 155: 171–184.

Cosgrove DJ (2005) Growth of the plant cell wall. Nature Reviews Molecular Cell Biology 6: 850–861.

Cosgrove DJ (2015a) Plant cell wall extensibility: connecting plant cell growth with cell wall structure, mechanics, and the action of wall modifying enzymes. Journal of Experimental Botany 67: 463–476.

Cosgrove DJ (2015b) Plant expansins: diversity and interactions with plant cell walls. Current Opinion in Plant Biology 25: 162–172.

Eklöf JM and Brumer H (2010) The XTH gene family: an update on enzyme structure, function, and phylogeny in xyloglucan remodelling. Plant Physiology 153: 456–466.

Franková L and Fry SC (2013) Biochemistry and physiological roles of enzymes that ‘cut and paste’ plant cell‐wall polysaccharides. Journal of Experimental Botany 64: 3519–3550.

Guerriero G, Fugelstad J and Bulone V (2010) What do we really know about cellulose biosynthesis in higher plants? Journal of Integrative Plant Biology 52: 161–175.

Harholt J, Suttangkakul A and Scheller HV (2010) Biosynthesis of pectin. Plant Physiology 153: 384–395.

Hall Q and Cannon MC (2002) The cell wall hydroxyproline‐rich glycoprotein RSH is essential for normal embryo development in Arabidopsis. The Plant Cell 14: 1–12.

Hematy K, Cherk C and Somerville S (2009) Host‐pathogen warfare at the plant cell wall. Current Opinion in Plant Biology 12: 406–413.

Hu H, Brown PH and Labavitch JM (1996) Species variability in boron requirement is correlated with cell wall pectin. Journal of Experimental Botany 47: 227–232.

Jamet E, Albenne C, Boudart G, et al. (2008) Recent advances in plant cell wall proteomics. Proteomics 8: 893–908.

Johnston SL, Prakash R, Chen NJ, et al. (2013) An enzyme activity capable of endotransglycosylation of heteroxylan polysaccharides is present in plant primary cell walls. Planta 237: 173–187.

Joseleau JP, Imai T, Kuroda K and Ruel K (2004) Detection in situ and characterization of lignin in the G‐layer of tension wood fibres of Populus deltoides. Planta 219: 338–345.

Lamport DT, Kieliszewski MJ, Chen Y and Cannon MC (2011) Role of the extensin superfamily in primary cell wall architecture. Plant Physiology 156: 11–19.

Lopez M, Bizot H, Chambat G, et al. (2010) Enthalpic studies of xyloglucan – cellulose interactions. Biomacromolecules 11: 1417–1428.

Miedes E, Zarra I, Hoson T, et al. (2011) Xyloglucan endotransglucosylase and cell wall extensibility. Journal of Plant Physiology 168: 196–203.

Mirabet V, Das P, Boudaoud A and Hamant O (2011) The role of mechanical forces in plant morphogenesis. Annual Review of Plant Biology 62: 365–385.

Mohnen D (2008) Pectin structure and biosynthesis. Current Opinion in Plant Biology 11: 266–277.

Nishikubo N, Takahashi J, Roos AA, et al. (2011) Xyloglucan endo‐transglycosylase‐mediated xyloglucan rearrangements in developingwood of hybrid aspen. Plant Physiology 155: 399–413.

Nixon BT, Mansouri K, Singh A, et al. (2016) Comparative structural and computational analysis supports eighteen cellulose synthases in the plant cellulose synthesis complex. Scientific Reports 6: 28696.

Pabst M, Fischl RM, Brecker L, et al. (2013) Rhamnogalacturonan II structure shows variation in the side chains monosaccharide composition and methylation status within and across different plant species. The Plant Journal 76: 61–72.

Panteris E, Adamakis ID, Daras G and Rigas S (2015) Cortical microtubule patterning in roots of Arabidopsis thaliana primary cell wall mutants reveals the bidirectional interplay with cell expansion. Plant Signaling & Behavior 10: e1028701.

Park YB and Cosgrove DJ (2015) Xyloglucan and its interactions with other components of the growing cell wall. Plant and Cell Physiology 56: 180–194.

Pelloux J, Rusterucci C and Mellerowicz EJ (2007) New insights into pectin methylesterase structure and function. Trends in Plant Science 12: 267–277.

Ringli C, Keller B and Ryser U (2001) Glycine‐rich proteins as structural components of plant cell walls Cell. Cellular and Molecular Life Sciences 58: 1430–1441.

del Rio LA and Pusso A (2009) Reactive Oxygen Species in Plant Signaling. Heidelberg: Springer Verlag.

Saladie M, Rose JK, Cosgrove DJ and Catala C (2006) Characterization of a new xyloglucan endotransglucosylase/hydrolase (XTH) from ripening tomato fruit and implications for the diverse modes of enzymic action. The Plant Journal 47: 282–295.

Sanchez‐Rodríguez C, Rubio‐Somoza I, Sibout R and Persson S (2010) Phytohormones and the cell wall in Arabidopsis during seedling growth. Trends in Plant Science 15: 291–301.

Scheller HV and Ulvskov P (2010) Hemicelluloses. Annual Review of Plant Biology 61: 263–289.

Schröder R, Wegrzyn TF, Bolitho KM and Redgwell RJ (2004) Mannan transglycosylase: a novel enzyme activity in cell walls of higher plants. Planta 219: 590–600.

Schultink A, Liu L, Zhu L and Pauly M (2014) Structural diversity and function of xyloglucan sidechain substituents. Plants 3: 526–542.

Seifert GJ and Roberts K (2007) The biology of arabinogalactan proteins. Annual Review of Plant Biology 58: 137–161.

Simmons TJ, Mortimer JC, Bernardinelli OD, et al. (2016) Folding of xylan onto cellulose fibrils in plant cell walls revealed by solid‐state NMR. Nature Communications 21: article 13902.

Tan L, Showalter A, Egelund J, et al. (2012) Arabinogalactan‐proteins and the research challenges for these enigmatic plant cell surface proteoglycans. Frontiers in Plant Science 3: e140.

Tan L, Eberhard S, Pattathil S, et al. (2013) An Arabidopsis cell wall proteoglycan consists of pectin and arabinoxylan covalently linked to an arabinogalactan protein. The Plant Cell 25: 270–287.

Vain T, Crowell EF, Timpano H, et al. (2014) The cellulase KORRIGAN is part of the cellulose synthase complex. Plant Physiology 165: 1521–1532.

Velasquez S, Ricardi MM, Dorosz JG, et al. (2011) O‐Glycosylated cell wall proteins are essential in root hair growth. Science 332: 1401–1403.

Zhong R and Ye ZH (2015) Secondary cell walls: biosynthesis, patterned deposition and transcriptional regulation. Plant and Cell Physiology 56: 195–214.

Further Reading

Morgan JL, Strumillo J and Zimmer J (2013) Crystallographic snapshot of cellulose synthesis and membrane translocation. Nature 493: 181–186.

Purushotham P, Cho SH, Díaz‐Moreno SM, et al. (2016) A single heterologously expressed plant cellulose synthase isoform is sufficient for cellulose microfibril formation in vitro. Proceedings of the National Academy of Sciences of the United States of America 113: 11360–11365.

Schuetz M, Benske A, Smith R, et al. (2014) Laccases direct lignification in the discrete secondary cell wall domains of protoxylem. Plant Physiology 166: 798–807.

Wolf S, Hematy K and Hofte H (2012) Growth control and cell wall signaling in plants. Annual Review of Plant Biology 63: 381–407.

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Srivastava, Vaibhav, McKee, Lauren S, and Bulone, Vincent(Jul 2017) Plant Cell Walls. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1002/9780470015902.a0001682.pub3]