Plant Gene Mapping Techniques

David Grant, Iowa State University, Ames, Iowa, USA
Randy C Shoemaker, Iowa State University, Ames, Iowa, USA

Published online: April 2001

DOI: 10.1038/npg.els.0002019

Plant genetic maps are graphic representations of the organization of chromosomes. Maps are developed using a wide range of classical and molecular techniques and population structures.

Keywords: genetic maps; comparative mapping; DNA markers; recombination; polyploid; aneuploid

Figure 1. Development of mapping populations. Crossing strategies for developing both RILs (recombinant inbred lines) and BCs (backcrosses) are shown.
Figure 2. Mapping with monosomics. Panels (a) and (b) represent an array of DNA samples. P1, P2 diploid parents; F1, progeny of P1 × P2; 1, , 7, progeny of P1 × monosomic derivatives of P2, each of which was missing a single chromosome. DNAs in each panel were hybridized with an RFLP (restriction fragment length polymorphism) probe with a unknown chromosomal location. The results indicate that the probe used in panel (a) came from chromosome 3 while the one used in panel (b) came from chromosome 6.
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 Further Reading
    Bennetzen J and Freeling M (1993) Grasses as a single genetic system: genome composition, collinearity and compatibility. Trends in Genetics 9: 250–252.
    book Caetano-Anolles G and Gresshoff PM (eds) (1997) DNA Markers: Protocols, Applications, and Overviews. New York: Wiley-Liss.
    book Silva J and Sorrells M (1996) "Linkage analysis in polyploids using molecular markers". In: Jauhas PP Methods of Genome Analysis in Plants, pp. 211–226. New York: CRC Press

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Plant Genetics and Molecular Biology
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Article Title: Plant Gene Mapping Techniques
 
How to Cite close
Grant, David, and Shoemaker, Randy C(Apr 2001) Plant Gene Mapping Techniques. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002019]