Plant Mutagenesis and Mutant Screening

Abstract

Plant mutagenesis is the induction of new genetic variants, frequently by treatment with chemicals, ionizing radiation, or mobilization of insertion elements. The new genetic variation may be used to give insight into almost any biological process.

Keywords: mutagenesis; somaclonal variation; epistasis

Figure 1.

The use of primers from the transposon and from the gene to identify an insertion in the gene of interest by PCR amplification. Usually the PCR product is sequenced to confirm the result. In the case of sequenced tagged collections, the sequencing from the primers in the insert is already done, and the results are stored in a searchable database.

Figure 2.

Linear biochemical pathway to demonstrate the simplest case of epistasis. A, B and C are metabolites whose concentrations are affected by the genes and the enzymes they encode.

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References

Avery L and Wasserman S (1992) Ordering gene function: the interpretation of epistasis in regulatory hierarchies. Trends in Genetics 8: 312–316.

Benson RJ, Johal GS, Crane VC et al. (1995) Cloning and characterization of the maize An1 gene. Plant Cell 7: 75–84.

Bhattacharyya MK, Smith AM, Ellis THN, Hedley C and Martin C (1990) The wrinkled‐seed character of pea described by Mendel is caused by a transposon‐like insertion in a gene encoding starch‐branching enzyme. Cell 60: 115–122.

Gilliland LU, McKinney EC, Asmussen MA and Meagher RB (1998) Detection of deleterious genotypes in multigenerational studies. I. Disruptions in individual Arabidopsis actin genes. Genetics 149: 717–725.

Haughn GW and Somerville CR (1987) Selection for herbicide resistance at the whole plant level. In: LeBaron HM (ed.) Biotechnology in Agricultural Chemistry, pp 98–108. Washington, DC: American Chemical Society.

Jacobsen SE and Meyerowitz EM (1997) Hypermethylated SUPERMAN epigenetic alleles in Arabidopsis. Science 277: 1100–1103.

Li SL and Rédei GP (1969) Estimation of mutation rate in autogamous diploids. Radiation Botany 9: 125–132.

McCourt P (1999) Genetic analysis of hormone signalling. Annual Review of Plant Physiology and Plant Molecular Biology 50: 219–243.

Mendel GJ (1865) Versuche über pflanzen‐hybriden. Verh. naturf. vereins Brünn 4: 3–47.

Nelson OE (1968) Waxy locus in maize. 2. Location of controlling element alleles. Genetics 60: 507–524.

Nelson OE (1987) The waxy locus in maize 25 years later. Genetics 116: 339–342.

Pelaz S, Ditta GS, Baumann E, Wisman E and Yanofsky MF (2000) B and C floral organ identity functions require SEPALLATA MADS‐box genes. Nature 405: 200–203.

Phillips PC (1998) The language of gene interaction. Genetics 149: 1167–1171.

Phillips RL, Kaeppler SM and Olhoft P (1994) Genetic instability of plant tissue cultures: breakdown of normal controls. Proceedings of the National Academy of Sciences of the USA 91: 5222–5226.

SanMiguel P, Tikhonov A, Jin YK et al. (1996) Nested retrotransposons in the intergenic regions of the maize genome. Science 274: 765–768.

Scanlon MJ, Chen KD and McKnight CC (2000) The narrow sheath duplicate genes: sectors of dual aneuploidy reveal ancestrally conserved gene functions during maize leaf development. Genetics 155: 1379–1389.

Silverstone AL, Mak PY, Martinez EC and Sun TP (1997) The new RGA locus encodes a negative regulator of gibberellin response in Arabidopsis thaliana. Genetics 146: 1087–1099.

Sparrow AH (1976) Comparison of X‐ray and gamma‐ray dose response curves for pink somatic mutations in Tradescantia clone‐02. Radiation and Environmental Biophysics 13: 295–303.

Stadler LJ (1928) Genetic effects of X‐rays in maize. Proceedings of the National Academy of Sciences of the USA 14: 69–75.

Vogel JP, Schuerman P, Woeste K, Brandsatter I and Kieber J (1998) Isolation and characterization of Arabidopsis mutants defective in the induction of ethylene biosynthesis by cytokinin. Genetics 149: 417–427.

Wagner A (2000) Robustness against mutations in genetic networks of yeast. Nature Genetics 24: 355–361.

Weil CF and Wessler SR (1990) The effects of plant transposable element insertion on transcription initiation and RNA processing. Annual Review of Plant Physiology and Plant Molecular Biology 41: 527–552.

Wessler SR and Varagona MJ (1985) Molecular‐basis of mutations at the waxy locus of maize: correlation with the fine‐structure genetic‐map. Proceedings of the National Academy of Sciences of the USA 82: 4177–4181.

Further Reading

Avery L and Wasserman S (1992) Ordering gene function: the interpretation of epistasis in regulatory hierarchies. Trends in Genetics 8: 312–316.

Bouché N and Bouchez D (2001) Arabidopsis gene knockout: phenotypes wanted. Current Opinion in Plant Biology 4: 111–117.

Evans DA (1989) Somaclonal variation: genetic‐basis and breeding applications. Trends in Genetics 5: 46–50.

Feldmann KA, Malmberg RL and Dean C (1994) Mutagenesis in Arabidopsis. In: Somerville CR and Meyerowitz EM (eds) Arabidopsis, pp. 137–172. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.

Krysan PJ, Young JC and Sussman MR (1999) T‐DNA as an insertional mutagen in Arabidopsis. Plant Cell 11: 2283–2290.

Malmberg RL (1993) Production and analysis of plant mutants emphasizing Arabidopsis thaliana. In: Glick BR and Thompson JE (eds) Methods in Plant Molecular Biology and Biotechnology, pp. 11–28. Boca Raton, FL: CRC Press.

Neuffer MG (1982) Mutant induction in maize. In: Sheridan WF (ed.) Maize for Biological Research, pp 61–64. Charlottesville, NC: Plant Molecular Biology Association.

Rédei GP (1975) Arabidopsis as a genetic tool. Annual Review of Genetics 9: 111–127.

Tautz D (2000) A genetic uncertainty problem. Trends in Genetics 16: 475–477.

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How to Cite close
Malmberg, Russell L(Jul 2003) Plant Mutagenesis and Mutant Screening. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002020]