Arabidopsis thaliana as an Experimental Organism


In the recent years Arabidopsis thaliana has undoubtedly emerged as the model experimental organism for essentially all aspects of plant biology, due to its short generation time, its small genome size and the availability of its complete deoxyribonucleic acid sequence. In addition, Arabidopsis has proven to be an ideal organism for studying plant development at the molecular, cellular, organismal and ecological level. By concentrating their efforts on a single model system, Arabidopsis scientists have made outstanding advances in almost any field of plant research. Further, discoveries made in Arabidopsis have been translated to other plant species such as economically important crops, as well as to animal systems, including complex human disease processes.

Key Concepts

  • Arabidopsis is a small flowering plant, which has emerged as the primary experimental organism for the study of all aspects of plant biology.

  • Arabidopsis genome has been fully sequenced in 2000 and contains approximately 27‚ÄČ000 genes.

  • Several molecular tools have been established to identify Arabidopsis transcription profiles, epigenetic landscape, protein and metabolite composition.

  • Gain of function and loss of function transgenic lines for specific Arabidopsis genes can be easily made or obtained from public collections.

  • Most of the information on Arabidopsis research is available from dedicated websites.

  • Discoveries made in Arabidopsis can be easily translated to other plant species, as well as to animal systems.

Keywords: Arabidopsis thaliana; plant development; reverese genetics; mutagenesis; transgenic; transcriptome analysis

Figure 1.

An approximately 4‐week‐old plant of the frequently used laboratory accession Landsberg erecta. Total plant height is at this stage 15 cm. The inset shows a single flower.

Figure 2.

Establishment of the Arabidopsis body plan in the embryo and the structure of a primary root. A, apical region; C, central region; B, basal region; HY, hypophyseal cell; SAM, shoot apical meristem; COT, cotyledon; H, hypocotyl; ER, embryonic root; RM, root meristem; RMI, root meristem initials.

Figure 3.

Gene functional analysis using reverse genetics tools. (a) Outline of the ‘reverse genetics’ strategy used for the functional analysis of genes of interest. (b) Examples of the application of reverse genetics tools to the functional study of small gene families regulating crucial developmental pathways in Arabidopsis. In Arabidopsis, two COP9 signalosome complex (CSN) subunits, CSN5 and CSN6, are both encoded by two highly conserved genes, named CSN5A and CSN5B, and CSN6A and CSN6B, respectively. The availability of T‐DNA insertion lines in each member of these small gene families has allowed the generation of the csn5a csn5b and csn6a cns6b double null mutants. In both cases, as shown in (b), complete loss of function of CSN5 or CSN6, results in severe developmental defects causing post‐embryonic arrest at the seedling stage ( reproduced from Gusmaroli et al., , Copyright American Society of Plant Biologists).



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Serino, Giovanna, and Gusmaroli, Giuliana(Jul 2011) Arabidopsis thaliana as an Experimental Organism. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1002/9780470015902.a0002031.pub2]