Zebrafish as an Experimental Organism

The zebrafish became, in the last decade, a model organism of choice to study vertebrate development. Outstanding studies in cell biology coupled with an increasing wealth in mutants and genomic tools led to an explosion of contributions from the zebrafish research community in understanding formation of the brain, heart and main organs.

Keywords: development; embryology; genetics; cell biology

Figure 1. (a) One-day-old zebrafish embryo, lateral view. (b) Dorsal view of the neurons of the pineal gland. (c) Expression pattern of an early neurogenic marker in the neural plate at the end of gastrulation (dorsal view). (d) Primary axonal tracts in a 2-day-old brain. (e) Lateral view of the zebrafish brain showing telencephalic emx-1 gene expression (blue) and transplanted cells in the eye (brown). (f) Rescue of a telencephalon (expressing emx-1 in blue) in a brain mutant after transplant of wild-type anterior neural plate cells (brown). (g) Transplanted kriesler mutant cells cannot contribute to rhombomere 5 and 6 in a wild-type embryo. Brown cells (g) are kreisler cells. Blue cells are rhombomere 3 and 5 krox-20-expressing cells. (g) is adapted from Moens et al. (1998).
Figure 2. (a–d) Lateral views of mutants affected in notochord and tail formation (a is wild-type). (e, f) Lateral view of the brain of the acerebellar mutation (f, arrow) compared to wild-type (e, arrow). Alcian blue staining of the jaw in wild-type (g) and a jaw mutant (h). Lateral view of a 5-day-old wild-type (i) and colourless pigment mutant (j) embryos. (k) Wild-type (top, arrow) and heart mutant (bottom, arrow) 5-day-old embryos. (l) Axonal staining (brown) of a forebrain mutant masterblind (left) and wild-type (right) 2-day-old embryos. All panels but (l) are adapted from the zebrafish issue, Development 123 (December 1996).
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 References
    Amacher S and Kimmel CB (1998) Promoting notochord fate and repressing muscle development in zebrafish axial mesoderm. Development 125: 1397–1406.
    Barresi MJ, Stickney HL and Devoto SH (2000) The zebrafish slow-muscle-omitted gene product is required for Hedgehog signal transduction and the development of slow muscle identity. Development 127: 2189–2199.
    Barth KA, Kishimoto Y, Rohr KB et al. (1999) Bmp activity establishes a gradient of positional information throughout the entire neural plate. Development 126: 4977–4987.
    book Detrich WH, Westerfield M and Zon LI (eds) (1999a) "The Zebrafish: Biology". Methods in Cell Biology, vol. 59. San Diego: Academic Press.
    book Detrich WH, Westerfield M and Zon LI (eds) (1999b) "The Zebrafish: Genetics and Genomics". Methods in Cell Biology, vol. 60. San Diego: Academic Press.
    Dooley K and Zon LI (2000) Zebrafish: a model system for the study of human disease. Current Opinion in Genetics and Development 10: 252–256.
    Drummond IA, Majumdar A, Hentschel H et al. (1998) Early development of the zebrafish pronephros and analysis of mutations affecting pronephric function. Development 125: 4655–4667.
    Eisen JS (1991) The role of interactions in determining cell fate of two identified motoneurons in the embryonic zebrafish. Neuron 8: 231–240.
    Fekany-Lee K, Gonzalez E, Miller-Bertoglio V and Solnica-Krezel L (2000) The homeobox gene bozozok promotes anterior neuroectoderm formation in zebrafish through negative regulation of BMP2/4 and Wnt pathways. Development 127: 2333–2345.
    Feldman B, Gates MA, Egan ES et al. (1998) Zebrafish organizer development and germ-layer formation require nodal-related signals. Nature 395: 181–185.
    Feldman B, Dougan ST, Schier AF and Talbot WS (2000) Nodal-related signals establish mesendodermal fate and trunk neural identity in zebrafish. Current Biology 10: 531–534.
    Griffin KJP, Amacher SL, Kimmel CB and Kimelman D (1998) Molecular identification of spadetail: regulation of zebrafish trunk and tail mesoderm function by T-box genes. Development 125: 3379–3388.
    Halpern ME, Hatta K, Amacher SL et al. (1997) Genetic interactions in zebrafish midline development. Developmental Biology 187: 154–170.
    Hatta K, Kimmel CB, Ho RK and Walker C (1991) The cyclops mutation blocks specification of the floor plate of the zebrafish central nervous system. Nature 350: 339–341.
    Heisenberg CP, Tada M, Rauch GJ et al. (2000) Silberblick/Wnt11 mediates convergent extension movements during zebrafish gastrulation. Nature 405: 76–81.
    Ho RK and Kane DA (1990) Cell-autonomous action of zebrafish spt-1 mutation in specific mesodermal precursors. Nature 348: 728–730.
    Houart C, Westerfield M and Wilson SW (1998) A small population of anterior cells patterns the forebrain during zebrafish gastrulation. Nature 391: 788–792.
    Karlstrom RO, Talbot WS and Schier AF (1999) Comparative syntony cloning of zebrafish you-too: mutations in the Hedgehog target gli2 affect ventral forebrain patterning. Genes and Development 13: 388–393.
    Kimmel CB, Hatta K and Eisen JS (1991) Genetic control of primary neurons development in zebrafish. Development supplement 2: 47–57.
    Kupperman E, Au S, Osborne N, Waldron S and Stainier DY (2000) A sphingosine-1-phosphate receptor regulator cell migration during vertebrate heart development. Nature 406: 192–195.
    Lun K and Brand M (1998) A series of no isthmus (noi) alleles of the zebrafish pax2.1 gene reveals multiple signaling events in development of the midbrain-hindbrain boundary. Development 125: 3049–3062.
    Macdonald R, Scholes J, Strahle U et al. (1997) The pax protein Noi is required for commissural axon pathway formation in the rostral forebrain. Development 124: 2397–2408.
    Masai I, Heisenberg CP, Barth KA, Macdonald R, Adamek S and Wilson SW (1997) Floating head and masterblind regulate neuronal patterning in the roof of the forebrain. Neuron 18: 43–57.
    Moens CB, Cordes SP, Giorgianni MW, Barsh GS and Kimmel CB (1998) Equivalence in the genetic control of hindbrain segmentation in fish and mouse. Development 125: 381–391.
    Reifers F, Bohli H, Walsh EC, Crossley PH, Stainier DY and Brand M (1998) Fgf8 is mutated in zebrafish acerebellar (ace) mutants and is required for maintenance of midbrain-hindbrain boundary development and somitogenesis. Development 125: 2381–2395.
    Striesinger G, Walker C, Dover N, Knauber D and Singer F (1981) Production of clones of homozygous diploid zebrafish. Nature 291: 293–296.
    Walker C and Striesinger G (1983) Induction of mutations by -rays in pre-gonial germ cells of zebrafish embryos. Genetics 103: 125–136.
    book Westerfield M (1995) The Zebrafish Book. Eugene, OR: University of Oregon Press.
    Zebrafish Issue (1996) Development 123: 1–460.
    Zhang J, Talbot WS and Schier AF (1998) Positional cloning identifies zebrafish one-eyed-pinhead as a permissive EFG-related ligand required during gastrulation. Cell 92: 241–251.
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Houart, Corinne(Apr 2001) Zebrafish as an Experimental Organism. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002094]