Cell Culture: Basic Procedures

Patricia Mericko, American Type Culture Collection, Manassas, Virginia, USA
Angela Helmirch, American Type Culture Collection, Manassas, Virginia, USA
LuPeng Chen, American Type Culture Collection, Manassas, Virginia, USA
Kenichi Kusamoto, American Type Culture Collection, Manassas, Virginia, USA
Araz Toumadje, American Type Culture Collection, Manassas, Virginia, USA
Christine Chapline, American Type Culture Collection, Manassas, Virginia, USA
Denry Sato, American Type Culture Collection, Manassas, Virginia, USA
David W Barnes, American Type Culture Collection, Manassas, Virginia, USA

Published online: May 2002

DOI: 10.1038/npg.els.0002556

Cell culture has provided a valuable research tool for over 100 years. Cell culture is a broad term applied to the wide range of techniques to culture cells on an artificial nutrient medium in a controlled culture environment.

Keywords: culture medium; primary cell culture; cell culture equipment; cryopreservation

 Further Reading
    book Barnes D and Sirbasku D (eds) (1987) "Peptide growth factors". Methods In Enzymology, vol. 146, part A; vol. 147, part B. New York: Academic Press.
    book Barnes D, Sirbasku D and Sato G (eds) (1984) Cell Culture Methods For Molecular and Cell Biology, 4 vols. New York: Wiley-Liss.
    book Barnes D, Mather J and Sato G (eds) (1991) "Peptide growth factors". Methods In Enzymology, vol. 198, part C. New York: Academic Press.
    book Butler MJ (1997) Animal Cell Culture and Technology. London: IRL Press.
    book Darling DC and Morgan SJ (1994) Animal Cells: Culture and Media. Chichester, UK: Wiley.
    book Darling DC and Morgan SJ (1994) Animal Cell Culture: Introduction to Biotechniques. Oxford: Bios.
    book Doyle D, Hay R and Kirsop BE (eds) (1991) Animal Cells: Living Resources for Biotechnology. Cambridge: Cambridge University Press.
    book Freshney RI (ed.) (1992) Animal Cell Culture: A Practical Approach. London: IRL Press.
    book Freshney RI (1994) Culture of Animal Cells: A Manual of Basic Techniques, 3rd edn. New York: Wiley-Liss.
    book Harrison MA and Rae IF (1997) General Techniques of Cell Culture (Handbooks in Practical Animal Cell Biology). Cambridge: Cambridge University Press.
    book Jakoby WB and Pastan IH (1979) "Cell culture". Methods in Enzymology, vol. 58. New York: Academic Press
    book Mather J and Barnes D (eds) (1998) "Animal cell culture". Methods In Cell Biology, vol. 57. New York: Academic Press.
    book Mather JP and Roberts PE (1998) Introduction to Cell and Tissue Culture: Theory and Technique. New York: Plenum.
    book Murakami H, Yamane I, Hayashi I et al. (eds) (1985) Growth and Differentiation of Cells in Defined Environments. Berlin: Springer.
    book Pollard JW and Walker JM (1990) "Animal Cell Culture". Methods in Molecular Biology, vol. 5. Totowa, NJ: Humana Press.
    book Various editors (1989–current) Proceedings of the Annual Meeting of the Japanese Association for Animal Cell Technology. Dordrecht: Kluwer.
    book Wasley JD and May JW (1971) Animal Cell Culture Methods. Philadelphia: Lippincott-Raven.

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Techniques in Cell Biology
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Article Title: Cell Culture: Basic Procedures
 
How to Cite close
Mericko, Patricia, Helmirch, Angela, Chen, LuPeng, Kusamoto, Kenichi, Toumadje, Araz, Chapline, Christine, Sato, Denry, and Barnes, David W(May 2002) Cell Culture: Basic Procedures. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002556]