Cell Culture Media


Cell culture media comprise the biochemical constituents required to sustain proliferation and biological function of cells in vitro.

Keywords: nutrient; intermediary metabolism; proliferation; bioproduction; serum‐free


Fike R, Kubiak J, Price P and Jayme D (1993) Feeding strategies for enhanced hybridoma productivity: automated concentrate supplementation. BioPharm 6: 49–54.

Ham RG and McKeehan WL (1979) Media and growth requirements. Methods in Enzymology 58: 44–93.

Jayme DW (1999) An animal origin perspective of common constituents of serum‐free medium formulations. Developments in Biological Standardization 99: 169–175.

Jayme DW and Blackman KE (1985) Culture media for propagation of mammalian cells, viruses and other biologicals. In: Mizrahi A and van Wezel AL (eds) Advances in Biotechnological Processes, vol. 5, pp. 1–30. New York: AR Liss.

Jayme DW and Gruber DF (1998) Development of serum‐free media and methods for optimization of nutrient composition. In: Celis JE (ed.) Cell Biology: A Laboratory Handbook, 2nd edn, vol. 1, pp. 19–26. San Diego: Academic Press.

Jayme DW, Watanabe T and Shimada T (1997) Basal medium development for serum‐free culture: a historical perspective. Cytotechnology 23: 95–101.

Waymouth C (1984) Preparation and use of serum‐free culture media. In: Methods for Preparation of Media, Supplements, and Substrata for Serum‐Free Animal Cell Culture, pp. 23–68. New York: AR Liss.

Further Reading

Bio Whittaker (1998) [http://www.biowhittaker.com]

Celis JE (ed.) (1988) Cell Biology: A Laboratory Handbook, 2nd edn. San Diego: Academic Press.

Freshney RI (ed.) (1987) Culture of Animal Cells: A Manual of Basic Technique, 2nd edn. New York: AR Liss.

HyClone Laboratories (1999) [http://www.hyclone.com]

Irvine Scientific (1999) [http://www.irvinesci.com]

JRH Biosciences (1997) [http://www.csl.com.au/jrh_div/jrh_indx.htm]

Life Technologies (1999) [http://lifetech.com]

Mather JR and Barnes D (eds) (1998) Methods in Cell Biology, vol. 57: Animal Cell Culture Methods. San Diego: Academic Press.

Media Tech (1999) [http://www.cellgro.com]

Sigma (2000) [http://www.sigma‐aldrich.com]

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How to Cite close
Jayme, David W(Apr 2001) Cell Culture Media. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002558]