Cell culture media comprise the biochemical constituents required to sustain proliferation and biological function of cells in vitro.
Keywords: nutrient; intermediary metabolism; proliferation; bioproduction; serum-free
Cell Culture Media
David W Jayme, Life Technologies Inc., Grand Island, New York, USA
Published online: April 2001
DOI: 10.1038/npg.els.0002558
| References | |
| Fike R, Kubiak J, Price P and Jayme D (1993) Feeding strategies for enhanced hybridoma productivity: automated concentrate supplementation. BioPharm 6: 49–54. | |
| Ham RG and McKeehan WL (1979) Media and growth requirements. Methods in Enzymology 58: 44–93. | |
| Jayme DW (1999) An animal origin perspective of common constituents of serum-free medium formulations. Developments in Biological Standardization 99: 169–175. | |
| book Jayme DW and Blackman KE (1985) "Culture media for propagation of mammalian cells, viruses and other biologicals". In: Mizrahi A and van Wezel AL (eds) Advances in Biotechnological Processes, vol. 5, pp. 1–30. New York: AR Liss. | |
| book Jayme DW and Gruber DF (1998) "Development of serum-free media and methods for optimization of nutrient composition". In: Celis JE (ed.) Cell Biology: A Laboratory Handbook, 2nd edn, vol. 1, pp. 19–26. San Diego: Academic Press. | |
| Jayme DW, Watanabe T and Shimada T (1997) Basal medium development for serum-free culture: a historical perspective. Cytotechnology 23: 95–101. | |
| book Waymouth C (1984) "Preparation and use of serum-free culture media". In: Methods for Preparation of Media, Supplements, and Substrata for Serum-Free Animal Cell Culture, pp. 23–68. New York: AR Liss. | |
| Further Reading | |
| ePath Bio Whittaker (1998) [http://www.biowhittaker.com] | |
| book Celis JE (ed.) (1988) Cell Biology: A Laboratory Handbook, 2nd edn. San Diego: Academic Press. | |
| book Freshney RI (ed.) (1987) Culture of Animal Cells: A Manual of Basic Technique, 2nd edn. New York: AR Liss. | |
| ePath HyClone Laboratories (1999) [http://www.hyclone.com] | |
| ePath Irvine Scientific (1999) [http://www.irvinesci.com] | |
| ePath JRH Biosciences (1997) [http://www.csl.com.au/jrh_div/jrh_indx.htm] | |
| ePath Life Technologies (1999) [http://lifetech.com] | |
| book Mather JR and Barnes D (eds) (1998) Methods in Cell Biology, vol. 57: Animal Cell Culture Methods. San Diego: Academic Press. | |
| ePath Media Tech (1999) [http://www.cellgro.com] | |
| ePath Sigma (2000) [http://www.sigma-aldrich.com] | |
