Cell Line Authentication Methods

Yvonne A Reid, American Type Culture Collection, Manassas, VA, USA
A Scott Durkin, American Type Culture Collection, Manassas, VA, USA
Kate Steenbergen, American Type Culture Collection, Manassas, VA, USA
Robert J Hay, American Type Culture Collection, Manassas, VA, USA

Published online: April 2001

DOI: 10.1038/npg.els.0002559

In vitro cell culture systems are crucial research tools for discerning the complex mechanisms and pathways that regulate cell growth, differentiation and development. The validity of conclusions drawn from this research demands unequivocal verification of cell line identity.

Keywords: DNA fingerprinting; cell line cross; contamination; mycoplasma detection; isoenzymology; cell culture

 References
    Durkin AS and Reid YA (1998) Short tandem repeat loci utilized in human cell line identification. ATCC Connection 18: 1–7.
    Edwards A, Hammond HA, Jin L, Caskey CT and Chakraborty R (1992) Genetic variation at five trimeric and tetrameric tandem repeat loci in four human population groups. Genomics 12: 241–253.
    Gilbert DA, Reid YA, Gail MH et al. (1990) Application of DNA fingerprints for cell-line individualization. American Journal of Human Genetics 47: 499–514.
    Halton DM, Peterson WD and Hukku B (1983) Cell culture quality control by rapid isoenzyme characterization. In Vitro 19: 16–24.
    book Harasawa R, Uemori T and Asada K (1993) "Sensitive detection of mycoplasmas in cell cultures by using two-step polymerase chain reaction". In: Kahane I and Adoni A (eds) Rapid Diagnosis of Mycoplasmas, pp. 227–232. New York: Plenum Press.
    book Hay RJ (1992a) "Cell line preservation and characterization". In: Freshney RI (ed.) Animal Cell Culture: A Practical Approach, pp. 95–148. Oxford: IRL.
    book Hay RJ (1992b) "Testing for microbial contaminants – bacteria and fungi". In: Hay RJ, Caputo J and Macy ML (eds) ATCC Quality Control Methods for Cell Lines, 2nd edn, pp. 19–33. Rockville, MD: American Type Culture Collection.
    book Hay RJ (1998) "Cell banking and authentication". In: Brown F, Griffiths E, Horaud JC and Petricciani JC (eds) Safety of Biological Products Prepared from Mammalian Cell Culture, Developments in Biological Standardization, 93, pp. 15–19. Basel: Karger.
    Hu M and Buck C (1993) Application of polymerase reaction technique for detection of mycoplasma contamination. Journal of Tissue Culture Methods 15: 155–160.
    Jeffreys AJ, Wilson V and Thein SL (1985) Hypervariable ‘minisatellite’ regions in human DNA. Nature 314: 67–73.
    Kaplan J and Hukku B (1998) Cell characterization and authentication. Methods in Cell Biology 57: 203–216.
    McGarrity GJ, Sarama J and Vanaman V (1985) Cell culture techniques. ASM News 51: 170–183.
    Nakamura Y, Leppert M, O'Connell P et al. (1987) Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616–1622.
    O’Brien SJ, Shannon JE and Gail HE (1980) A molecular approach to the identification and individualization of human and animal cells in culture: isozyme and allozyme genetic signatures. In Vitro 16: 119–134.
    Rottem S and Barile MF (1993) Beware of mycoplasmas. Trends in Biotechnology 11: 143–150.
 Further Reading
    Chen TR (1977) In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Experimental Cell Research 104: 255–262.
    McGarrity GJ (1976) Spread and control of mycoplasmal infection of cell cultures. In Vitro 12: 643–648.
    book Lincoln CK and Gabridge MA (1998) "Cell culture contamination: sources, consequences, prevention and elimination". In: Mather JP and Barnes (eds) Animal Cell Culture Methods, pp. 50–64. New York: Academic Press.
    Nelson-Rees WA, Flandermeyer RR and Hawthorne PK (1975) Distinctive banded marker chromosomes of human tumor cell lines. International Journal of Cancer 16: 74–82.
    Peterson WD, Simpson WF and Hukku B (1979) Cell culture characterization: monitoring for cell identification. Methods in Enzymology 58: 164–178.

Related Sub-Topics:

Techniques in Cell Biology | Techniques and Tools in Molecular Biology | Biochemical and Biophysical Techniques
Contact Editor close
Submit a note to the editor about this article by filling in the form below.

* Required Field

Article Title: Cell Line Authentication Methods
 
How to Cite close
Reid, Yvonne A, Durkin, A Scott, Steenbergen, Kate, and Hay, Robert J(Apr 2001) Cell Line Authentication Methods. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002559]