Animal Cell Culture Scale‐up

Eunan McGlinchey, Dublin City University, Dublin, Ireland

Published online: January 2007

DOI: 10.1002/9780470015902.a0002562.pub2

Cultures of animal cells can be grown in vitro within controlled laboratory environments and are used for research in cell biology or as a method for producing products of commercial interest. Various methods for scale-up of these cultures are available.

Keywords: animal cell; cell culture; scale-up; bioreactor; suspension cells

Figure 1. Types of bioreactors for industrial use. (a) Photo showing 10 L bioreactor culture system (glass bioreactor). (b) Photo showing 100 L bioreactor culture system (stainless-steel bioreactor). (c) Fluidized bed bioreactor. From Butler (2004). Reproduced with permission from Thomson. (d) Airlift bioreactor. From Butler (2004). Reproduced with permission from Thomson. (e) Packed bed bioreactor. From Butler (2004). Reproduced with permission from Thomson. (f) Wave bioreactor.
Figure 2. Animal cell culture scale-up technologies. (a) Photo showing culture flasks, spinner flask and roller bottle technologies. (b) Nunc multitray unit. (c) Costar Cell Cube bioreactor. Reprinted with permission from Aunins et al. (2003). © 2003 American Chemical Society & American Institute of Chemical Engineers. (d) Hollow fibre systems. From Butler (2004). Reproduced with permission from Thomson.
close
 References
    Arathoon WR and Birch JR (1986) Large-scale cell culture in biotechnology. Science 232: 1390–1395.
    Aunins JG, Bader B, Caola A et al. (2003) Fluid mechanics, cell distribution, and environment in CellCube bioreactors. Biotechnology Progress 19(1): 2–8.
    other Birch JR (1997) Review of biotechnology-derived products in use and in development. European Journal of Parenteral Sciences. Science Biotechnology Special Issue 3–10.
    book Butler M (2004) Animal Cell Culture and Technology, 2nd edn. Oxford and New York: Garland Science/Bios Scientific Publishers.
    other Cartwright T (1997) Biotechnology-based manufacturing methods for bulk-purified pharmaceutical proteins. European Journal of Parenteral Sciences. Science Biotechnology Special Issue 11–18.
    other Dream RF (1993) Bioreactors in bioprocessing. Pharmaceutical Engineering (November/December): 56–66.
    book Griffiths JB (1999) "Mammalian cell culture reactors, Scale-up". In: Flickinger MC and Drew SW (eds) Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation, vol. 3, pp. 1594–1607. Chichester: Wiley
    Kretzmer G (2002) Industrial processes with animal cells.Applied Microbiology and Biotechnology 59: 135–142.
    Walsh G (2003) Pharmaceutical biotechnology products approved within the European Union. European Journal of Pharmaceutics and Biopharmaceutics 55: 3–10.
    Wurm FM (2004) Production of recombinant protein therapeutics in cultivated mammalian cells. Nature Biotechnology 22(11): 1393–1398.
 Further Reading
    book Doyle A and Griffiths JB (eds) (1998) Culture processes and scale-up. Cell and Tissue Culture: Laboratory Procedures in Biotechnology. Chichester: Wiley.
    book Doyle A, Griffiths JB and Newell DG (eds) (1993) Upstream processing. Cell and Tissue Culture: Laboratory Procedures, pp. 28.1–28E:3.21. Chichester: Wiley.
    book Freshney RI (2000) Culture of Animal Cells: a Manual of Basic Technique, 4th edn. Chichester: Wiley.
    book Griffiths B (1992) "Scaling-up of animal cell cultures". Freshney RI (ed.) Animal Cell Culture: A Practical Approach, pp. 47–93. Oxford: IRL Press
    book Lubiniecki AS (ed.) (1990) "Large-scale Mammalian Cell Culture Technology", vol. 10. Bioprocess Technology. New York: Marcel Dekker.
    book Spier RE (ed.) (2000) Encyclopedia of Cell Technology, vol. 1. New York: Wiley.

Related Sub-Topics:

Techniques in Cell Biology
Contact Editor close
Submit a note to the editor about this article by filling in the form below.

* Required Field

Article Title: Animal Cell Culture Scale‐up
 
How to Cite close
McGlinchey, Eunan(Jan 2007) Animal Cell Culture Scale‐up. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0002562.pub2]