Animal Cell Culture Scale‐up

Abstract

Cultures of animal cells can be grown in vitro within controlled laboratory environments and are used for research in cell biology or as a method for producing products of commercial interest. Various methods for scale‐up of these cultures are available.

Keywords: animal cell; cell culture; scale‐up; bioreactor; suspension cells

Figure 1.

Types of bioreactors for industrial use. (a) Photo showing 10 L bioreactor culture system (glass bioreactor). (b) Photo showing 100 L bioreactor culture system (stainless‐steel bioreactor). (c) Fluidized bed bioreactor. From Butler . Reproduced with permission from Thomson. (d) Airlift bioreactor. From Butler . Reproduced with permission from Thomson. (e) Packed bed bioreactor. From Butler . Reproduced with permission from Thomson. (f) Wave bioreactor.

Figure 2.

Animal cell culture scale‐up technologies. (a) Photo showing culture flasks, spinner flask and roller bottle technologies. (b) Nunc multitray unit. (c) Costar Cell Cube bioreactor. Reprinted with permission from Aunins et al. . © 2003 American Chemical Society & American Institute of Chemical Engineers. (d) Hollow fibre systems. From Butler . Reproduced with permission from Thomson.

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References

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Aunins JG, Bader B, Caola A et al. (2003) Fluid mechanics, cell distribution, and environment in CellCube bioreactors. Biotechnology Progress 19(1): 2–8.

Birch JR (1997) Review of biotechnology‐derived products in use and in development. European Journal of Parenteral Sciences. Science Biotechnology Special Issue 3–10.

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Griffiths JB (1999) Mammalian cell culture reactors, Scale‐up. In: Flickinger MC and Drew SW (eds) Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation, vol. 3, pp. 1594–1607. Chichester: Wiley

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Wurm FM (2004) Production of recombinant protein therapeutics in cultivated mammalian cells. Nature Biotechnology 22(11): 1393–1398.

Further Reading

Doyle A and Griffiths JB (eds) (1998) Culture processes and scale‐up. Cell and Tissue Culture: Laboratory Procedures in Biotechnology. Chichester: Wiley.

Doyle A, Griffiths JB and Newell DG (eds) (1993) Upstream processing. Cell and Tissue Culture: Laboratory Procedures, pp. 28.1–28E:3.21. Chichester: Wiley.

Freshney RI (2000) Culture of Animal Cells: a Manual of Basic Technique, 4th edn. Chichester: Wiley.

Griffiths B (1992) Scaling‐up of animal cell cultures. Freshney RI (ed.) Animal Cell Culture: A Practical Approach, pp. 47–93. Oxford: IRL Press

Lubiniecki AS (ed.) (1990) Large‐scale Mammalian Cell Culture Technology, vol. 10. Bioprocess Technology. New York: Marcel Dekker.

Spier RE (ed.) (2000) Encyclopedia of Cell Technology, vol. 1. New York: Wiley.

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How to Cite close
McGlinchey, Eunan(Jan 2007) Animal Cell Culture Scale‐up. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0002562.pub2]