Michel Pagé, Laval University, Quebec City, Quebec, Canada
Published online: April 2001
DOI: 10.1038/npg.els.0002568
Drug development and screening of natural products for their cytotoxicity require methods that are reliable, rapid, sensitive and economic. The growth of mammalian cells in microplates is used to monitor the cytotoxicity of potentially active compounds.
Keywords: cytotoxicity; assays; screening; in vitro
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Figure 1. Absorption spectra of Alamar Blue in reduced and nonreduced forms. The compound was reduced by addition of sodium borohydride.
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Figure 2. Standard fluorescence intensity curve for SKOV 3 cells incubated with Alamar Blue.
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Figure 3. Percent inhibition at various concentrations of daunorubicin in the cytotoxicity assay with Alamar Blue.
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| References | |
| Borenfreund E and Puerner JA (1985) Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicology Letters 24: 199–124. | |
| Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65: 55–63. | |
| Pagé B and Pagé M (1995) Sensitive colorimetric cytotoxicity measurement using Alamar Blue. Oncology Reports 2: 59–61. | |
| Pagé B, Pagé M and Noël C (1993) A new fluorometric assay for cytotoxicity measurements in vitro. International Journal of Oncology 3: 473–476. | |
| Scudiero DA, Shoemaker RH, Paull KD et al. (1988) Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture. Cancer Research 48: 4827–4833. | |
| Further Reading | |
| book Pagé M (1997) "High-volume screening". In Teicher BA Anticancer Drug Development Guide, chap. I, pp 3–21. Totowa, NJ: Humana Press. | |
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