Yeast Cell Culture

Yeasts – unicellular, eukaryotic fungi with membrane-bound nucleus and mitochondria – were among the first microorganisms to be domesticated. They are still used for brewing and baking, but under appropriate culture conditions they also find numerous technological applications, including fodder production, production of fermented foods, waste disposal and, when genetically engineered, the production of vaccines and therapeutic proteins.

Keywords: yeast; Saccharomyces cerevisiae; brewing; baker¢s yeast; biotechnology; food yeasts; continuous culture

Figure 1. (a) Diagram of a conventional stirred fermenter for cultivation of yeasts. A, stirrer, with speed control; B, baffles; C, sparger, for introduction of air or oxygen; D, ports for addition of antifoam and acid or alkali for pH control; E, sampling port. (b) Diagram of stirred fermenter, with medium reservoirs (S), feed and harvesting pumps (F) and spent culture receiver.
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 References
    book Bui and Galzy (1990) "Food yeasts". In: Spencer JFT and Spencer DM (eds) Yeast Technology, pp. 241–265. Berlin: Springer-Verlag.
    book Evans IH (1990) "Yeast strains for baking". In: Spencer JFT and Spencer DM (eds) Yeast Technology, pp. 13–54. Berlin: Springer-Verlag.
    book Fleet GH (ed.) (1993) Wine Microbiology and Technology. Amsterdam: Harwood Academic Publishers.
    book Higgins DR and Cregg JM (1997) Pichia Protocols. Methods in Molecular Biology, vol. 103. Totowa, NJ: Humana Press.
    book Kurtzmann CP and Fell JW (eds) (1998) The Yeasts: a Taxonomic Study. Elsevier: Amsterdam.
    book Spencer JFT and Spencer DM (eds) (1990) Yeast Technology. Berlin: Springer-Verlag.
 Further Reading
    book Burrows S (1979) "Baker's yeast". In: Rose AH (ed.) Microbial Biomass. London: Academic Press.
    book Evans IH (ed.) (1996) Yeast Protocols. Totowa, NJ: Humana Press.
    book Rose AH (ed.) (1978) Primary Products of Metabolism. London: Academic Press.
    book Rose AH and Harrison JS (eds) (1987) The Yeasts, vol 1: Biology of Yeasts, 2nd edn. London: Academic Press.
    book Rose AH and Harrison JS (eds) (1987) The Yeasts, vol 2: Yeasts and the Environment, 2nd edn. London: Academic Press.
    book Rose AH and Harrison JS (eds) (1989) The Yeasts, vol 3: Metabolism and Physiology of Yeasts, 2nd edn. London: Academic Press.
    book Rose AH and Harrison JS (eds) (1991) The Yeasts, vol 4: Yeast Organelles, 2nd edn. London: Academic Press.
    book Spencer DM and Spencer JFT (1997) Yeasts in Natural and Artificial Habitats. Berlin: Springer-Verlag.
    book Spencer JFT, Spencer DM and Smith ARW, (1983) Yeast Genetics: Fundamental and Applied Aspects. Berlin: Springer-Verlag.
    book Walton EF and Yarranton GT (eds) (1989) Molecular and Cellular Biology of Yeasts. Glasgow, UK: Blackie & Son Ltd.
    book Zimmermann FK and Entian KD (eds) (1997) Yeast Sugar Metabolism. Lancaster, PA, USA: Technomic Publishing.
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de Spencer, Alicia l Ragout, and Spencer, JFT(Feb 2003) Yeast Cell Culture. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002578]