Plant Cell Culture


Plant cell culture is a broad term applied to a wide range of techniques involving plant parts cultured on an artificial nutrient medium under a controlled culture environment.

Keywords: plant tissue culture; in vitro culture; micropropagation; clonal propagation; plant genetic engineering; virus‐free plants; embryo rescue; haploid plants; secondary products

Figure 1.

Boston fern runner tip approximately 4 cm long. After surface disinfestation, the Clorox burned end is cut off and the tip is placed in culture.



Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473–497.

Smith RH (2000) Plant Tissue Culture Techniques and Experiments, 2nd edn. San Diego, CA: Academic Press.

Further Reading

Burr RW (1976) Mass propagation of ferns through tissue culture. In Vitro 12: 209–269.

Cooke RC (1977) The use of an agar substitute in the initial growth of Boston ferns in vitro. HortScience 12: 339.

Kyte L and Kleyn J (1996) Plants from Test Tubes: An Introduction to Micropropagation, 3rd edn. Portland, OR: Timber Press.

Oki L (1981) The modification of research procedures for commercial propagation of Boston ferns. Environmental and Experimental Botany 21: 397–413.

Pedhya MA and Mehta AR (1982) Propagation of fern (Nephrolepsis) through tissue culture. Plant Cell Reports 1: 261–263.

Roberts DJ (1965) Modern propagation of ferns. Proceedings of the International Plant Propagation Society 15: 317–321.

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How to Cite close
Smith, Roberta H(Apr 2001) Plant Cell Culture. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1038/npg.els.0002581]