Roberta H Smith, Texas A&M University, College Station, Texas, USA
Published online: April 2001
DOI: 10.1038/npg.els.0002581
Plant cell culture is a broad term applied to a wide range of techniques involving plant parts cultured on an artificial nutrient medium under a controlled culture environment.
Keywords: plant tissue culture; in vitro culture; micropropagation; clonal propagation; plant genetic engineering; virus-free plants; embryo rescue; haploid plants; secondary products
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Figure 1. Boston fern runner tip approximately 4 cm long. After surface disinfestation, the Clorox burned end is cut off and the tip is placed in culture.
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| References | |
| Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473–497. | |
| book Smith RH (2000) Plant Tissue Culture Techniques and Experiments, 2nd edn. San Diego, CA: Academic Press. | |
| Further Reading | |
| Burr RW (1976) Mass propagation of ferns through tissue culture. In Vitro 12: 209–269. | |
| Cooke RC (1977) The use of an agar substitute in the initial growth of Boston ferns in vitro. HortScience 12: 339. | |
| book Kyte L and Kleyn J (1996) Plants from Test Tubes: An Introduction to Micropropagation, 3rd edn. Portland, OR: Timber Press. | |
| Oki L (1981) The modification of research procedures for commercial propagation of Boston ferns. Environmental and Experimental Botany 21: 397–413. | |
| Pedhya MA and Mehta AR (1982) Propagation of fern (Nephrolepsis) through tissue culture. Plant Cell Reports 1: 261–263. | |
| Roberts DJ (1965) Modern propagation of ferns. Proceedings of the International Plant Propagation Society 15: 317–321. | |
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