Adenovirus Culture


Adenovirus culture is the propagation of adenovirus in tissue culture for subsequent use for basic and applied virus research.

Keywords: virology; tissue culture; gene therapy


Crystal RG (1999) In vivo and ex vivo gene therapy strategies to treat tumors using adenovirus gene transfer vectors. Cancer Chemotherapy and Pharmacology 43 (supplement): S90–S99.

Flint J and Shenk T (1997) Viral transactivating proteins. Annual Review of Genetics 31: 177–212.

Horwitz MS (1996) Adenoviruses. In: Fields BN, Knipe DM, Howley PM et al. (eds) Fields Virology, 3rd edn, pp. 2149–2171. Philadelphia: Lippincott‐Raven.

Mittereder N, March KL and Trapnell BC (1996) Evaluation of the concentration and bioactivity of adenovirus vectors for gene therapy. Journal of Virology 70: 7498–7509.

Tollefson AE, Hermiston TW and Wold WSM (1999) Preparation and titration of CsCl‐banded adenovirus stocks. In: Wold WSM (ed.) Adenovirus Methods and Protocols, pp. 1–9. Totowa, NJ: Humana Press.

Wold WSM and Chinnadurai G (2000) Adenovirus proteins that regulate apoptosis. In: Cann AJ (ed.) DNA Virus Replication, pp. 200–232. Oxford, UK: Oxford University Press.

Wold WSM, Doronin K, Toth K et al. (1999) Immune responses to adenoviruses: viral evasion mechanisms and their implications for the clinic. Current Opinion in Immunology 11: 380–386.

Further Reading

Doerfler W and Bohm P (eds) (1995) The molecular repertoire of adenoviruses. Current Topics in Microbiology and Immunology 199/III.

Seth P (ed.) (1999) Adenoviruses: Basic Biology to Gene Therapy. Austin, TX: Landes.

Shenk T (1996) Adenoviridae: the viruses and their replication. In: Fields BN, Knipe DM, Howley PM et al. (eds) Fields Virology 3rd edn, pp. 2111–2148. Philadelphia: Lippincott‐Raven.

White E (1998) Regulation of apoptosis by adenovirus E1A and E1B oncogenes. Seminars in Virology 8: 505–513.

Wold WSM (ed.) (1999) Adenovirus Methods and Protocols. Totowa, NJ: Humana Press.

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Wold, William SM, and Tollefson, Ann E(Apr 2001) Adenovirus Culture. In: eLS. John Wiley & Sons Ltd, Chichester. [doi: 10.1038/npg.els.0002583]