Salvatore T Butera, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
Published online: April 2001
DOI: 10.1038/npg.els.0002587
To successfully isolate and propagate retroviruses, several critical aspects of viral tropism and replication must be considered. Issues related to viral growth can be quite varied among the retroviral family members and dictate the establishment of appropriate culture conditions.
Keywords: cellular activation; viral replication; primary or transformed cells; viral tropism; cocultures
| References | |
| Hammer S, Crumpacker C, D'Aquila R et al. (1993) Use of virologic assays for detection of human immunodeficiency virus in clinical trials: recommendations of the AIDS clinical trials group virology committee. Journal of Clinical Microbiology 31: 2557–2564. | |
| Heneine W, Switzer WM, Sandstrom P et al. (1998) Identification of a human population infected with simian foamy viruses. Nature Medicine 4: 403–407. | |
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| Further Reading | |
| book Cossart YE (1999) "Virus isolation versus molecular methods". In: Lennette EH and Smith TF (eds) Laboratory Diagnosis of Viral Infections, 3rd edn. New York: Marcel Dekker. | |
| book Crowe SM and Mills J (1999) "Human immunodeficiency viruses: HIV-1 and HIV-2". In: Lennette EH and Smith TF (eds) Laboratory Diagnosis of Viral Infections, 3rd edn. New York: Marcel Dekker. | |
| Flügel RF (1991) Spumaviruses: a group of complex retroviruses. Journal of Acquired Immune Deficiency Syndromes 4: 739–750. | |
| Kitamura K, Rudolph DR, Goldsmith C, Folks TM and Lal RB (1993) Isolation, characterization, and transmission of human T-lymphotropic virus Types I and II in culture. Current Microbiology 27: 355–360. | |
| book Kucera LS and Myrvik QN (1985) Retroviruses. In: Fundamentals of Medical Virology, 2nd edn. Philadelphia: Lea and Febiger. | |
| book Yamamoto N and Hinuma Y (1999) "Human T-lymphotropic viruses I and II". In: Lennette EH and Smith TF (eds) Laboratory Diagnosis of Viral Infections, 3rd edn. New York: Marcel Dekker. | |
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