Plasma Membranes: Methods for Preparation

Abstract

All cells are contained within plasma membranes, which have important functions in the cell's relation with its surroundings. Plasma membranes can be purified from different kinds of tissues and cells in order to study their structure and function.

Keywords: plasma membrane; centrifugation; two‐phase partitioning; affinity partitioning

Further Reading

Aronson NN Jr and Touster O (1974) Isolation of rat liver plasma membrane fragments in isotonic sucrose. Methods in Enzymology 31: 90–102.

Larsson C, Sommarin M and Widell S (1994) Isolation of highly purified plant plasma membranes and separation of inside‐out and right‐side‐out vesicles. Methods in Enzymology 228: 451–469.

Mircheff AK (1989) Isolation of plasma membranes from polar cells and tissues: apical/basolateral separation, purity, function. Methods in Enzymology 172: 18–34.

Persson A and Jergil B (1995) The purification of membranes by affinity partitioning. FASEB Journal 9: 1304–1310.

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How to Cite close
Jergil, Bengt, and Ekblad, Lars(Apr 2001) Plasma Membranes: Methods for Preparation. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002589]