Yanzhuang Wang, University of Michigan, Ann Arbor, Michigan, USA
Published online: July 2008
DOI: 10.1002/9780470015902.a0002590.pub2
The Golgi apparatus can be purified from cells and tissues using a number of different methods, depending on the purpose for which it is to be used. Purified Golgi membranes have been used in a variety of cell-free assays to investigate sugar modifications, vesicle transport, structural formation of the Golgi apparatus and the interaction of the Golgi with microtubules.
Keywords: Golgi apparatus; fractionation; cell-free assays
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Figure 1. Representative micrographs of purified Golgi membranes in low (a) and high (b) magnifications.
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| References | |
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| Further Reading | |
| Bell AW, Ward MA, Blackstock WP et al. (2001) Proteomics characterization of abundant Golgi membrane proteins. The Journal of Biological Chemistry 276: 5152–5165. | |
| Chabin-Brion K, Marceiller J, Perez F et al. (2001) The Golgi complex is a microtubule-organizing organelle. Molecular Biology of the Cell 12: 2047–2060. | |
| Efimov A, Kharitonov A, Efimova N et al. (2007) Asymmetric CLASP-dependent nucleation of noncentrosomal microtubules at the trans-Golgi network. Developmental Cell 12: 917–930. | |
| Mogelsvang S and Howell KE (2006) Global approaches to study Golgi function. Current Opinion in Cell Biology 18: 438–443. | |
| Shorter J and Warren G (2002) Golgi architecture and inheritance. Annual Review of Cell and Developmental Biology 18: 379–420. | |
| Wang Y, Wei JH, Bisel B, Tang D and Seemann J (2008) Golgi cisternal unstacking stimulates COPI vesicle budding and protein transport. PLoS ONE 3: e1647. | |
| Ye B, Zhang Y, Song W et al. (2007) Growing dendrites and axons differ in their reliance on the secretory pathway. Cell 130: 717–729. | |
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