Synaptic vesicles are small organelles that store and release neurotransmitters at synapses. They can be purified from the brain or other sources to study their structure and function.
Keywords: neurotransmitter storage; organelles; synapse; brain
Synaptic Vesicles: Methods for Preparation
Erik Floor, University of Kansas, Lawrence, Kansas, USA
Published online: September 2005
DOI: 10.1038/npg.els.0003963
| References | |
| Belfort GM and Kandror KV (2003) Cellugyrin and synaptogyrin facilitate targeting of synaptophysin to a ubiquitous synaptic vesicle sized compartment in PC12 cells. Journal of Biological Chemistry Aug 18 [Epub ahead of print]. | |
| Berg EA, Johnson RJ, Leeman SE et al. (2000) Isolation and characterization of substance P-containing dense core vesicles from rabbit optic nerve and termini. Journal of Neuroscience Research 62: 830–839. | |
| Blumstein J, Faundez V, Nakatsu F et al. (2002) The neuronal form of adaptor protein-3 is required for synaptic vesicle formation from endosomes. Journal of Neuroscience 21: 8034–8042. | |
| Dauer W and Przedborski S (2003) Parkinson's disease: mechanisms and models. Neuron 39: 889–909. | |
| Floor E and Feist BE (1989) Most synaptic vesicles isolated from rat brain carry three membrane proteins, SV2, synaptophysin, and p65. Journal of Neurochemistry 52: 1433–1437. | |
| Floor E, Leventhal PS, Wang Y, Meng L and Chen W (1995) Dynamic storage of dopamine in rat brain synaptic vesicles in vitro. Journal of Neurochemistry 64: 689–699. | |
| Holroyd P, Lang T, Wenzel D, De Camilli P and Jahn R (2002) Imaging direct, dynamin-dependent recapture of fusing secretory granules on plasma membrane lawns from PC12 cells. Proceedings of the National Academy of Sciences of the USA 99: 16806–16811. | |
| Jin H, Wu H, Osterhaus G et al. (2003) Demonstration of functional coupling between -aminobutyric acid (GABA) synthesis and vesicular GABA transport into synaptic vesicles. Proceedings of the National Academy of Sciences of the USA 100: 4293–4298. | |
| Malinin VS, Frederic P and Lentz BR (2002) Osmotic and curvature stress affect PEG-induced fusion of lipid vesicles but not mixing of their lipids. Biophysical Journal 82: 2090–2100. | |
| Pothos EN, Mosharov E, Liu KP et al. (2002) Stimulation-dependent regulation of the pH, volume and quantal size of bovine and rodent secretory vesicles. Journal of Physiology (London) 542: 453–476. | |
| Takamori S, Riedel D and Jahn R (2000) Immunoisolation of GABA-specific synaptic vesicles defines a functionally distinct subset of synaptic vesicles. Journal of Neuroscience 20: 4904–4911. | |
| Further Reading | |
| book Hell JW and Jahn R (1994) "Preparation of synaptic vesicles from mammalian brain". Celis JE (ed.) Cell Biology: A Laboratory Handbook, pp. 567–574. New York: Academic Press. | |
| Murthy VN and De Camilli P (2003) Cell biology of the presynaptic terminal. Annual Review of Neuroscience 26: 701–728. | |
| Parsons SM (2000) Transport mechanisms in acetylcholine and monoamine storage. FASEB Journal 14: 2423–2434. | |
