Proteasomes: Methods for Preparation

Keiji Tanaka, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan

Published online: April 2001

DOI: 10.1038/npg.els.0002599

The 26S proteasome is a eukaryotic, ATP-dependent protease complex comprising the S20 proteasome and a pair of symmetrically disposed PA 700 regulatory particles. Both components can be purified by various chromatographic techniques after sample preparation.

Keywords: ubiquitin; proteolysis; ATP; 20S proteasome; 26S proteasome

 References
    Coux O, Tanaka K and Goldberg AL (1996) Structure and functions of the 20S and 26S proteasomes. Annual Review of Biochemistry 65: 801–847.
    Baumeister W, Walz J, Zühl F and Seemüller E (1998) The proteasome: paradigm of a self-compartmentalizing protease. Cell 92: 367–380.
    Bochtler M, Ditzel L, Groll M, Hartmann C and Huber H (1999) The proteasome. Annual Review of Biophysics and Biomolecular Structure 28: 295–317.
    Hochstrasser M (1996) Ubiquitin-dependent protein degradation. Annual Review of Genetics 30: 405–439.
    Hershko A and Ciechanover A (1998) The ubiquitin system. Annual Review of Biochemistry 67: 425–479.
    book Tanaka K and Tanahashi N (1997) "Preparation of proteasomes". In: Celis JE (ed.) Cell Biology: A Laboratory Handbook, 2nd edn, pp. 129–134. New York: Academic Press.
    Voges D, Zwickl P and Baumeister W (1999) The 26S proteasome: a molecular machine designed for controlled proteolysis. Annual Review of Biochemistry 68: 1015–1068.

Related Sub-Topics:

Cell Compartments and Cellular Organelles | Techniques in Cell Biology
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Article Title: Proteasomes: Methods for Preparation
 
How to Cite close
Tanaka, Keiji(Apr 2001) Proteasomes: Methods for Preparation. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002599]