Ribosomes: Methods for Preparation
Hendrik A Raué, Vrije Universiteit, Amsterdam, The Netherlands
Published online: May 2005
DOI: 10.1038/npg.els.0003964
Abstract
Ribosomes, the large protein–RNA complexes involved in translation of nucleotide sequences into proteins, are obtained from
cells broken open by grinding, shear forces or enzymatically. They are purified from the cell lysate by centrifugation to
remove cell debris, followed by sucrose density gradient centrifugation.
Keywords: ribosome; polysome; ribosomal subunits; sucrose density gradient
Further Reading
Bommer U,
Burkhardt N and
Jünemann R et al.
(1997)
Ribosomes and polysomes.
In: Graham J and
Rickwood D (eds)
Subcellular Fractionation: A Practical Approach,
pp. 271–301.
Washington, DC: IRL Press.
Garrett RA,
Douthwaite SR and
Liljas A et al.
(2000)
The Ribosome: Structure, Function, Antibiotics and Cellular Interactions.
Washington DC: American Society for Microbiology. [Conference proceedings.]
Matheson AT,
Davies JE,
Dennis PP and
Hill WE
(1995)
Frontiers in translation.
Biochemistry and Cell Biology
73: 739–1227.[Conference proceedings.].
Spector DL,
Goldman RD and
Leinwand LA
(1998)
Purification of ribosomes, ribosomal subunits, and polysomes.
In: Cells: A Laboratory Manual, part II: Culture and Biochemical Analysis of Cells.
Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
The Ribosome,
(2001)
Cold Spring Harbor Symposia on Quantitative Biology.
vol. LXVI
Cold Spring Harbor: Cold Spring Harbor Press. [Conference proceedings.].
Tuite MF,
Stansfield I and
Planta RJ
(1998)
Identifying genes encoding components of the protein synthesis machinery of the yeast Saccharomyces cerevisiae.
Methods in Microbiology
26: 351–373.