DNA: Methods for Preparation

Abstract

The isolation of DNA is required for genetic analysis and genetic engineering. There are primarily two types of method for isolating genomic DNA: (i) proteinase K digestion in the presence of SDS and EDTA at temperatures between 50 and 60°C; (ii) the use of strong chaotropic reagents such as guanidinium chloride. The trend is towards methods that are amenable to high‐throughput screening and automation.

Keywords: DNA; genomic; isolation; genetic analysis; genetic engineering

References

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Cheng S, Chen Y, Monforte JA, Higuchi R and Van Houten B (1995) Template integrity is essential of PCR amplification of 20–30 kb sequences from genomic DNA. PCR Methods and Applications 4: 294–298.

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Further Reading

Bignell GR, Miller AR and Evans IH (1996) Isolation of mitochondrial DNA. Methods in Molecular Biology 53: 109–116.

Cooper A and Wayne R (1998) New uses for old DNA. Current Opinion in Biotechnology 9: 49–53.

Dieffenbach CW and Dveksler GS (1995) PCR Primer. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.

Evans WE and Relling MV (1999) Pharmacogenomics: translating functional genomics into rational therapeutics. Science 286: 487–491.

Rapley R (2000) Nucleic Acid Protocols Handbook. Totowa, NJ: Humana Press.

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How to Cite close
Ling, Michael Mingfu(Apr 2001) DNA: Methods for Preparation. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002606]