Cell Invasion Assays

Claus Holst‐Hansen, Copenhagen University Hospital, Copenhagen, Denmark
Thomas Leth Frandsen, Copenhagen University Hospital, Copenhagen, Denmark
Nils Brünner, Copenhagen University Hospital, Copenhagen, Denmark

Published online: April 2001

DOI: 10.1038/npg.els.0002609

Cell invasion assays have been developed for quantitative study under well-established in vitro conditions of the ability of cultured cells to penetrate biological barriers such as artificial basement membrane.

Keywords: tumour cell culture; invasion; matrigel; transwell

Figure 1. Transwell invasion assay. The Transwell filter is coated with Matrigel (1). Cells, suspended in culture medium, are added to the upper chamber and culture medium to the lower chamber (2), and then incubated at 37°C for a defined period. The number of viable cells is quantified by the MTT method (3) and the resulting formazan crystals (4) in the two compartments are each dissolved in isopropanol and separately quantified colorimetrically (OD, optical density).
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 References
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Related Sub-Topics:

Cell Biology of Cancer | Techniques in Cell Biology
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Article Title: Cell Invasion Assays
 
How to Cite close
Holst‐Hansen, Claus, Frandsen, Thomas Leth, and Brünner, Nils(Apr 2001) Cell Invasion Assays. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002609]