Bernard B Allan, The Scripps Research Institute, La Jolla, California, USA
Jacques T Weissman, The Scripps Research Institute, La Jolla, California, USA
William E Balch, The Scripps Research Institute, La Jolla, California, USA
Published online: April 2001
DOI: 10.1038/npg.els.0002614
Newly synthesized cargo proteins translocated into the endoplasmic reticulum (ER) are incorporated into small vesicular carriers that mediate transport to the Golgi, via pre-Golgi intermediates along microtubule tracks. In vitro assays allow for the examination of factors that are required for and/or regulate the budding of vesicular carriers from the ER and the subsequent fusion of these vesicles with the Golgi.
Keywords: endoplasmic reticulum; golgi apparatus; vesicles; cell-free assay
| References | |
| Aridor M, Bannykh SI, Rowe T and Balch WE (1995) Sequential coupling between CopII and CopI vesicle coats in endoplasmic reticulum to Golgi transport. Journal of Cell Biology 131: 875–893. | |
| Beckers CJM, Keller DS and Balch WE (1987) Semi-intact cells permeable to macromolecules: use in reconstitution of protein transport from the endoplasmic reticulum to the Golgi complex. Cell 50: 523–534. | |
| Conradt B, Haas A and Wickner W (1994) Determination of four biochemically distinct, sequential stages during vacuole inheritance in vitro. Journal of Cell Biology 126: 99–110. | |
| Davidson HW and Balch WE (1993) Differential inhibition of multiple vesicular transport steps between the endoplasmic reticulum and trans Golgi network. Journal of Biological Chemistry 268: 4216–4226. | |
| Lafay F (1974) Envelope viruses of vesicular stomatitis virus: effect of temperature-sensitive mutations in complementation groups III and V. Journal of Virology 14: 1220–1228. | |
| Rexach MF and Schekman RW (1991) Distinct biochemical requirements for the budding, targeting, and fusion of ER-derived transport vesicles. Journal of Cell Biology 114: 219–229. | |
| Rowe T, Aridor M, McCaffery JM, Plutner H and Balch WE (1996) COPII vesicles derived from mammalian endoplasmic reticulum (ER) microsomes recruit COPI. Journal of Cell Biology 135: 895–911. | |
| Rowe T, Dascher C, Bannykh SI, Plutner H and Balch WE (1998) Role of vesicle-associated syntaxin 5 in the assembly of pre-Golgi intermediates. Science 279: 696–700. | |
| Further Reading | |
| book Allan BB and Balch WE (1999) "In vitro reconstruction of endoplasmic reticulum to Golgi transport". In: Bonafacino JS, Dasso M, Harford JB, Lippincott-Schwartz J and Yamada KM (eds) Current Protocols in Cell Biology, Unit 11.3. New York: John Wiley & Sons. | |
| Allan BB and Balch WE (1999) Protein sorting by directed maturation of Golgi compartments. Science 285: 63–66. | |
| book Allan BB, Weissman J, Aridor M et al. (2000) Stage specific assays to study cargo selection and role of SNAREs in export from the endoplasmic reticulum and delivery to the Golgi. Methods: A Companion to Methods in Enzymology (in press). | |
| Aridor M and Balch WE (1999) Integration of endoplasmic reticulum signaling in health and disease. Nature Medicine 5: 745–751. | |
| Nichols BJ and Pelham HR (1998) SNAREs and membrane fusion in the Golgi apparatus. Biochimica et Biophysica Acta 1404: 9–31. | |
| Palade GE (1975) Intracellular aspects of the process of protein transport. Science 189: 347–354. | |
| Rothman JE and Wieland FT (1996) Protein sorting by transport vesicles. Science 272: 227–234. | |
| Springer S, Spang A and Schekman R (1999) A primer on vesicle budding. Cell 97: 145–148. | |
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