Endoplasmic Reticulum to Golgi Transport: Methods


Newly synthesized cargo proteins translocated into the endoplasmic reticulum (ER) are incorporated into small vesicular carriers that mediate transport to the Golgi, via pre‐Golgi intermediates along microtubule tracks. In vitro assays allow for the examination of factors that are required for and/or regulate the budding of vesicular carriers from the ER and the subsequent fusion of these vesicles with the Golgi.

Keywords: endoplasmic reticulum; golgi apparatus; vesicles; cell‐free assay


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Further Reading

Allan BB and Balch WE (1999) In vitro reconstruction of endoplasmic reticulum to Golgi transport. In: Bonafacino JS, Dasso M, Harford JB, Lippincott‐Schwartz J and Yamada KM (eds) Current Protocols in Cell Biology, Unit 11.3. New York: John Wiley & Sons.

Allan BB and Balch WE (1999) Protein sorting by directed maturation of Golgi compartments. Science 285: 63–66.

Allan BB, Weissman J, Aridor M et al. (2000) Stage specific assays to study cargo selection and role of SNAREs in export from the endoplasmic reticulum and delivery to the Golgi. Methods: A Companion to Methods in Enzymology (in press).

Aridor M and Balch WE (1999) Integration of endoplasmic reticulum signaling in health and disease. Nature Medicine 5: 745–751.

Nichols BJ and Pelham HR (1998) SNAREs and membrane fusion in the Golgi apparatus. Biochimica et Biophysica Acta 1404: 9–31.

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Rothman JE and Wieland FT (1996) Protein sorting by transport vesicles. Science 272: 227–234.

Springer S, Spang A and Schekman R (1999) A primer on vesicle budding. Cell 97: 145–148.

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Allan, Bernard B, Weissman, Jacques T, and Balch, William E(Apr 2001) Endoplasmic Reticulum to Golgi Transport: Methods. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002614]