Immunoglobulin Purification

Abstract

Immunoglobulins are widely used as research and diagnostic tools and for passive immunization and treatment of immunocompromised disease states. Most applications require purification of the immunoglobulins; for example, clinical‐grade material must be of very high purity. The purification method of choice is dictated by the level of purity required and the intended use.

Keywords: immunoglobulin; purification; antibody; precipitation; chromatography

References

Cohn EJ, Gurd FRN, Surgenor DM et al. (1950) A system for the separation of the components of human blood: quantitative procedures for the separation of the protein components of human plasma. Journal of the American Chemical Society 72: 465–473.

Johnstone A and Thorpe R (1996) Immunochemistry in Practice, 3rd edn. Oxford: Blackwell Scientific.

Page M and Thorpe R (1996a) Purification of IgG using caprylic acid. In: Walker JM (ed.) The Protein Protocols Handbook, p. 723. Totawa, NJ: Humana Press.

Page M and Thorpe R (1996b) Purification of IgG using DEAE‐Sepharose chromatography. In: Walker JM (ed.) The Protein Protocols Handbook, p. 725. Totawa, NJ: Humana Press.

Page M and Thorpe R (1996c) Purification of IgG using Ion‐exchange HPLC/FPLC. In: Walker JM (ed.) The Protein Protocols Handbook, p. 727. Totawa, NJ: Humana Press.

Page M and Thorpe R (1996d) Purification of IgG using gel‐filtration chromatography. In: Walker JM (ed.) The Protein Protocols Handbook, p. 735. Totawa, NJ: Humana Press.

Page M and Thorpe R (1996e) Purification of IgG using affinity chromatography on antigen‐ligand columns. In: Walker JM (ed.) The Protein Protocols Handbook, p. 739. Totawa, NJ: Humana Press.

Page M and Thorpe R (1996f) Purification of IgG using protein A or protein G. In: Walker JM (ed.) The Protein Protocols Handbook, p. 733. Totawa, NJ: Humana Press.

Page M and Thorpe R (1996g) Purification of IgG using thiophilic chromatography. In: Walker JM (ed.) The Protein Protocols Handbook, p. 743. Totawa, NJ: Humana Press.

Page M and Thorpe R (1996h) Purification of IgG by precipitation with polyethylene glycol (PEG). In: Walker JM (ed.) The Protein Protocols Handbook, p. 731. Totawa, NJ: Humana Press.

Page M and Thorpe R (1998a) IgG purification. In: Pound JD (ed.) Methods in Molecular Biology. Immunochemical Protocols, pp. 95–111. Totawa, NJ: Humana Press

Page M and Thorpe R (1998b) Purification of monoclonal antibodies. In: Pound JD (ed.) Methods in Molecular Biology. Immunochemical Protocols, pp. 113–119. Totawa, NJ: Humana Press

Further Reading

Kerr MA and Thorpe R (1994) Immunochemistry LabFAX. Oxford: BIOS and Blackwell Scientific.

Pound JD (ed.) (1998) Methods in Molecular Biology: Immunochemical Protocols. Totawa, NJ: Humana Press.

Walker JM (ed.) (1996) The Protein Protocols Handbook. Totawa NJ: Humana Press.

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How to Cite close
Page, Mark(Apr 2001) Immunoglobulin Purification. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1038/npg.els.0002623]