Mark Page, Medeva Development, London, UK
Published online: April 2001
DOI: 10.1038/npg.els.0002623
Immunoglobulins are widely used as research and diagnostic tools and for passive immunization and treatment of immunocompromised disease states. Most applications require purification of the immunoglobulins; for example, clinical-grade material must be of very high purity. The purification method of choice is dictated by the level of purity required and the intended use.
Keywords: immunoglobulin; purification; antibody; precipitation; chromatography
| References | |
| Cohn EJ, Gurd FRN, Surgenor DM et al. (1950) A system for the separation of the components of human blood: quantitative procedures for the separation of the protein components of human plasma. Journal of the American Chemical Society 72: 465–473. | |
| book Johnstone A and Thorpe R (1996) Immunochemistry in Practice, 3rd edn. Oxford: Blackwell Scientific. | |
| book Page M and Thorpe R (1996a) "Purification of IgG using caprylic acid". In: Walker JM (ed.) The Protein Protocols Handbook, p. 723. Totawa, NJ: Humana Press. | |
| book Page M and Thorpe R (1996b) "Purification of IgG using DEAE-Sepharose chromatography". In: Walker JM (ed.) The Protein Protocols Handbook, p. 725. Totawa, NJ: Humana Press. | |
| book Page M and Thorpe R (1996c) "Purification of IgG using Ion-exchange HPLC/FPLC". In: Walker JM (ed.) The Protein Protocols Handbook, p. 727. Totawa, NJ: Humana Press. | |
| book Page M and Thorpe R (1996d) "Purification of IgG using gel-filtration chromatography". In: Walker JM (ed.) The Protein Protocols Handbook, p. 735. Totawa, NJ: Humana Press. | |
| book Page M and Thorpe R (1996e) "Purification of IgG using affinity chromatography on antigen-ligand columns". In: Walker JM (ed.) The Protein Protocols Handbook, p. 739. Totawa, NJ: Humana Press. | |
| book Page M and Thorpe R (1996f) "Purification of IgG using protein A or protein G". In: Walker JM (ed.) The Protein Protocols Handbook, p. 733. Totawa, NJ: Humana Press. | |
| book Page M and Thorpe R (1996g) "Purification of IgG using thiophilic chromatography". In: Walker JM (ed.) The Protein Protocols Handbook, p. 743. Totawa, NJ: Humana Press. | |
| book Page M and Thorpe R (1996h) "Purification of IgG by precipitation with polyethylene glycol (PEG)". In: Walker JM (ed.) The Protein Protocols Handbook, p. 731. Totawa, NJ: Humana Press. | |
| book Page M and Thorpe R (1998a) "IgG purification". In: Pound JD (ed.) Methods in Molecular Biology. Immunochemical Protocols, pp. 95–111. Totawa, NJ: Humana Press | |
| book Page M and Thorpe R (1998b) "Purification of monoclonal antibodies". In: Pound JD (ed.) Methods in Molecular Biology. Immunochemical Protocols, pp. 113–119. Totawa, NJ: Humana Press | |
| Further Reading | |
| book Kerr MA and Thorpe R (1994) Immunochemistry LabFAX. Oxford: BIOS and Blackwell Scientific. | |
| book Pound JD (ed.) (1998) Methods in Molecular Biology: Immunochemical Protocols. Totawa, NJ: Humana Press. | |
| book Walker JM (ed.) (1996) The Protein Protocols Handbook. Totawa NJ: Humana Press. | |
Copyright © 2000-2013 by John Wiley & Sons, Inc., or related companies. All rights reserved.
