Epitope Mapping: B‐cell Epitopes


Immunoglobulin molecules are folded to present a surface structure complementary to a surface feature on the antigen – the epitope is this feature of the antigen. Epitope mapping is the process of locating the antibody‐binding site on the antigen, although the term is also applied more broadly to receptor–ligand interactions unrelated to the immune system.

Keywords: antigen; antibody; phage display; mass spectrometry; site‐directed mutagenesis; immunoassay


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Further Reading

Morris GE (ed.) (1996) Epitope Mapping Protocols, Methods in Molecular Biology, vol. 66. Totowa, NJ: Humana Press. [A laboratory manual with practical details of all methods described here].

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How to Cite close
Morris, GE(Sep 2007) Epitope Mapping: B‐cell Epitopes. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0002624.pub2]