Epitope Mapping: B‐cell Epitopes

GE Morris, Keele University, Keele, Staffordshire, UK

Published online: September 2007

DOI: 10.1002/9780470015902.a0002624.pub2

Abstract

Immunoglobulin molecules are folded to present a surface structure complementary to a surface feature on the antigen – the epitope is this feature of the antigen. Epitope mapping is the process of locating the antibody‐binding site on the antigen, although the term is also applied more broadly to receptor–ligand interactions unrelated to the immune system.

Keywords: antigen; antibody; phage display; mass spectrometry; site‐directed mutagenesis; immunoassay

References

Barlow DJ, Edwards MS and Thornton JM (1986) Continuous and discontinuous protein antigenic determinants. Nature 322: 747–748.

Berzoksky JA (1985) Intrinsic and extrinsic factors in protein antigenic structure. Science 219: 932–940.

Flower DR (2003) Towards in silico prediction of immunogenic epitopes. Trends in Immunology 24: 667–674.

Hager‐Braun C and Tomer KB (2005) Determination of protein‐derived epitopes by mass spectrometry. Expert Review of Proteomics 2: 745–756.

Haste Andersen P, Nielsen M and Lund O (2006) Prediction of residues in discontinuous B‐cell epitopes using protein 3D structures. Protein Science 15: 2558–2567.

Pereboeva LA, Pereboev AV, Wang LF and Morris GE (2000) Hepatitis C epitopes from phage‐displayed cDNA libraries and their assembly into improved chimeric antigens. Journal of Medical Virology 60: 144–151.

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Wilson DS, Keefe AD and Szostak JW (2001) The use of mRNA display to select high‐affinity protein‐binding peptides. Proceedings of the National Academy of Sciences of the USA 98: 3750–3755.

Further Reading

Morris GE (ed.) (1996) Epitope Mapping Protocols, Methods in Molecular Biology, vol. 66. Totowa, NJ: Humana Press. [A laboratory manual with practical details of all methods described here].

Related Sub-Topics:

Diagnostics, Therapeutics and Methods | Proteins: Structure, Function, Metabolism | Protein Structure | Biomolecular Interactions | Antibodies
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Article Title: Epitope Mapping: B‐cell Epitopes
 
How to Cite close
Morris, GE(Sep 2007) Epitope Mapping: B‐cell Epitopes. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0002624.pub2]